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December 11, 2018

Webinar: A new paradigm in DNA sequencing – Highly accurate single-molecule long reads

In this webinar, Jonas Korlach, PacBio Chief Scientific Officer, and Dave Corney, Associate Principal Scientist, Next Generation Sequencing from GENEWIZ, describe the recent release of Sequel System 6.0, which has revolutionized long-read sequencing by providing users the ability to generate highly accurate single-molecule reads. Users no longer need to compromise read length for accuracy, because it is now possible to have both including Sanger-quality reads as long as 15 kb. They share the benefits in applications such as whole genome sequencing, structural variant detection, targeted sequencing and RNA sequencing of full-length transcripts using the Iso-Seq method. From those new to…

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October 16, 2018

ASHG PacBio Workshop: PacBio product updates and roadmap – announcing the release of new chemistry and software

In this ASHG workshop presentation , Jonas Korlach, CSO of PacBio, walked attendees through recent product updates and the coming technology roadmap. The Sequel System 6.0 release offered major improvements to accuracy, throughput, structural variant calling, and large-insert libraries, he said, showing examples of 35 kb libraries. Looking ahead, Korlach said that the V2 express library preparation product should be available early in 2019, with the new 8M SMRT Cell being introduced sometime later.

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October 16, 2018

ASHG PacBio Workshop: The Iso-Seq method for discovering alternative splicing in human diseases

In this ASHG workshop presentation, Elizabeth Tseng of PacBio showed how the Iso-Seq method can be used to discover disease-associated alternative splicing. Because this approach to isoform sequencing yields accurate, full-length transcripts requiring no assembly, it’s ideal for disease studies that need a more comprehensive picture of alternative splicing activity. Tseng offered several published examples of how the Iso-Seq method has been used for everything from single-gene studies to whole-transcriptome studies, and also detailed how the latest Sequel System chemistry recovers more genes and produces more usable reads.

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