Asset Tag: CCS
Application Brochure AAV Sequencing with HiFi Reads
With Single Molecule, Real-Time (SMRT®) sequencing on the Sequel® IIe systems, you can sequence AAV
genome populations to identify truncation, mutation, and host integration events. The AAV workflow from
PacBio® accommodates both scAAV and ssAAV constructs with easy on-instrument HiFi read generation
Procedure & Checklist – No-Amp Targeted Sequencing Utilizing the CRISPR-Cas9 System
Procedure & Checklist – Preparing 10 kb Library Using SMRTbell Express Template Prep Kit 2.0 for Metagenomics Shotgun Sequencing
Quick Reference Card – Loading and Pre-Extension Recommendations for the Sequel II and IIe Systems
Procedure & Checklist – Preparing HiFi Libraries from Low DNA Input Using SMRTbell Express Template Prep Kit 2.0
Procedure & Checklist – Preparing SMRTbell Libraries using PacBio Barcoded Overhang Adapters for Multiplexing Amplicons
Procedure & Checklist – Preparing HiFi SMRTbell Libraries using SMRTbell Express Template Prep Kit 2.0
Product Brochure: SMRT Link – Explore and analyze your data with confidence
With SMRT Link you can unlock the power of PacBio Single Molecule, Real-Time (SMRT) Sequencing using our portfolio of software tools designed to set up and monitor sequencing runs, review performance metrics, analyze, visualize, and annotate your sequencing data.
Application Brief: Targeted sequencing for amplicons – Best Practices
With Single Molecule, Real-Time (SMRT) Sequencing and the Sequel System, you can easily and cost effectively generate highly accurate long reads (HiFi reads, >99% single-molecule accuracy) from genes or regions of interest ranging in size from several hundred base pairs to 20 kb. Target all types of variation across relevant genomic regions, including low complexity regions like repeat expansions, promoters, and flanking regions of transposable elements.
Application Brochure: Gene editing validation with HiFi reads
With highly accurate long reads (HiFi reads) from the Sequel IIe System, powered by Single Molecule, Real-Time (SMRT) Sequencing technology, you can efficiently and cost effectively validate gene editing techniques including adeno-associated virus (AAV) and CRISPR-Cas9 approaches.
Application Brief: Whole genome sequencing for de novo assembly – Best Practices
PacBio HiFi reads provide both long read lengths (up to 25 kb) and high accuracy (>99.9%) to quickly and affordably generate contiguous, complete, and correct de novo genome assemblies of even the most complex genomes.