Application: Whole genome sequencing
August 1, 2024
How can I use HiFi bioinformatics to uncover germline variation?
Watch our video on HiFi sequencing bioinformatics for uncovering germline variation. It explains how HiFi reads provide a more complete view of the genome, introduces SMRTLink and open-source GitHub tools…
May 6, 2024
Does PacBio have a fully automated workflow solution?
PacBio has fully automated workflows for DNA extraction, DNA shearing, whole genome library prep, and targeted workflows.
May 6, 2024
Which library prep kit should I use for preparing libraries for Microbial WGS on HiFi systems?
HiFi plex prep kit 96. This kit and protocol offer the most cost efficient and streamlined prep for doing microbial WGS on HiFi systems.
May 6, 2024
Which library prep kit should I use for human, plant, or animal WGS on HiFi sequencing systems?
24 reaction kit: SMRTbell prep kit 3.0 (102-182-700) 96 reaction kit: HiFi prep kit 96 (103-381-200) Both kits and protocols use the same enzymatic steps and have qualified automated methods…
December 18, 2023
Where can I find an overview of all HiFi applications?
Here is an overview of HiFi applications
December 13, 2023
Which protocol do I need to follow to multiplex microbial libraries for WGS?
You can use the HiFi plex prep kit 96 and this protocol for multiplex microbial WGS and shotgun metagenomics applications
December 13, 2023
Where can I find more information on specific applications?
Explore our Training resources to access on-demand webinars, e-modules, and other educational materials. You can also visit our Sequencing Methods page to learn more about applications such as whole genome…
December 13, 2023
What types of genomic variants can be detected from PacBio HiFi data?
PacBio HiFi reads can detect single-nucleotide variants (SNVs), small indels (<20 bp in length) and structural variants (SVs), such as insertions, deletions, duplications, inversions, translocations, short tandem repeats (STRs), and…
December 13, 2023
How much DNA or cDNA do I need to do HiFi sequencing?
For genomic or metagenomic DNA, see page 1 of the following: Procedure & checklist – Preparing whole genome and metagenome libraries using SMRTbell prep kit 3.0 For 16S, see page…