Application: Targeted sequencing
April 24, 2024
For PureTarget, can I use samples that are not human blood and cell line extracted with Nanobind?
The supported sample types for PureTarget are Nanobind-extracted blood, cell line, and saliva. Please see table 4 in the app note (https://www.pacb.com/wp-content/uploads/Application-note-Comprehensive-genotyping-with-the-PureTarget-repeat-expansion-panel-and-HiFi-sequencing.pdf) for more details about other extraction kits and…
April 24, 2024
What guidance do you have for adding a small number of guide RNA pairs to the fixed PureTarget panel?
Please see the Tech note on custom PureTarget panels for more details.
April 24, 2024
Can I do a custom PureTarget panel?
Yes. Please see the Tech note on custom PureTarget panels for more details.
December 18, 2023
Where can I find an overview of all HiFi applications?
Here is an overview of HiFi applications
December 14, 2023
Should I do PCR or probe-based hybridization for my targeted sequencing project?
Typically, customers with many samples find it cost-effective to design a probe panel. View the Application brief – HiFi target enrichment best practices.
December 13, 2023
Where can I find more information on specific applications?
Explore our Training resources to access on-demand webinars, e-modules, and other educational materials. You can also visit our Sequencing Methods page to learn more about applications such as whole genome…
December 13, 2023
What types of genomic variants can be detected from PacBio HiFi data?
PacBio HiFi reads can detect single-nucleotide variants (SNVs), small indels (<20 bp in length) and structural variants (SVs), such as insertions, deletions, duplications, inversions, translocations, short tandem repeats (STRs), and…
December 13, 2023
Can I use PacBio sequencing systems for targeted sequencing?
Yes, we offer PureTarget panels for PCR free targeted sequencing, hybrid capture workflows with our partners, and amplicon sequencing. Visit the targeted sequencing page for further information.