Application: Microbiome + Metagenomics
May 6, 2024
Which library prep kit should I use for preparing libraries for Microbial WGS on HiFi systems?
HiFi plex prep kit 96. This kit and protocol offer the most cost efficient and streamlined prep for doing microbial WGS on HiFi systems.
December 18, 2023
Where can I find an overview of all HiFi applications?
Here is an overview of HiFi applications
December 13, 2023
Which protocol do I need to follow to multiplex microbial libraries for WGS?
If you are planning to barcode multiple microbial genomes and sequence them on a SMRT Cell, we recommend using our most recent chemistry and library preparation version, which is described…
December 13, 2023
Where can I find more information on specific applications?
You can find additional information on our training where you can search for and download online webinars, e-modules, and other training documents. You can also find various applications (WGS, targeted,…
December 13, 2023
What types of genomic variants can be detected from PacBio HiFi data?
PacBio HiFi reads can detect single-nucleotide variants (SNVs), small indels (<20 bp in length) and structural variants (SVs), such as insertions, deletions, duplications, inversions, translocations, short tandem repeats (STRs), and…
December 13, 2023
Can I use PacBio sequencing systems for targeted sequencing?
Yes. For general information about Targeted sequencing, please visit the targeted sequencing page, the PacBio Compatible partner targeted workflows page, or contact our technical support or field application specialists if…
December 13, 2023
How much DNA or cDNA do I need to do HiFi sequencing?
For genomic or metagenomic DNA, see page 1 of the following: Procedure & checklist – Preparing whole genome and metagenome libraries using SMRTbell prep kit 3.0 For 16S, see page…