HiFi data combine long read lengths (typically 15–25 kb) with Q30+ accuracy, they can be used directly in many standard bioinformatics workflows: De novo genome assembly – Produces highly contiguous assemblies with…
HiFi sequencing does not require PCR amplification because it is a single-molecule, real-time (SMRT) sequencing technology. Instead of copying DNA millions of times before sequencing, HiFi reads are generated by…
Outputs are delivered in standardized BAM format, ready for genome assembly, variant detection, phasing, and other downstream analyses.
The total time from DNA sample to HiFi reads typically ranges from a few days, depending on the application and system used. DNA extraction: ~2 hours using Nanobind kits Library preparation: ~1 day …
HiFi sequencing offers a streamlined, end-to-end workflow—from sample to analysis. Start with high-molecular-weight DNA extraction using Nanobind DNA kits, delivering quality DNA from a wide range of sample types in…
HiFi sequencing is the only known long-read technology to provide 99.9% or better accuracy in every run. With over 1,000 peer-reviewed publications in 2023 alone and over 1,200 HiFi sequencers…
HiFi sequencing now supports flexible, accessible input requirements: Whole genome sequencing: 500 ng DNA (SPRQ chemistry) Low-input workflows (Ampli-Fi): 1 ng DNA Isoform sequencing: 300 ng total RNA Single-cell cDNA: as little as 15…
The cost gap between long-read and short-read sequencing has narrowed significantly. Human whole genome sequencing (20× coverage) ~$345 per human genome on the Revio system with SPRQ-Nx chemistry, using as…
HiFi sequencing detects methylation directly from native DNA, automatically, in every run—no extra assays required. It works by measuring subtle changes in polymerase kinetics as modified bases (such as 5mC) are incorporated during sequencing. …
While multiple platforms offer long reads, HiFi stands out for: High per-read accuracy Each read is highly accurate on its own—reducing false positives and simplifying downstream analysis. Lower coverage requirements ~20× coverage…
HiFi sequencing combines long reads (500–20,000 bases) with 99.9% read accuracy, delivering comprehensive, high-confidence genomic and epigenomic insight in a single run. Unlike short reads (typically 150–200 bp), HiFi reads can span: Structural variants Repetitive…
No. PacBio does not charge for using SMRT Link Cloud. All core features, including run design, instrument monitoring, and run setup, are completely free with no usage limits. You are…
SMRT Link Cloud includes the same core features for instrument management, run setup, and monitoring as the local version of SMRT Link. However, SMRT Link Cloud does not include SMRT…
SMRT Link Cloud does not store your sequencing data. All data generated by your PacBio instruments is transferred to a storage location that you choose, such as your own cloud…
Your data stays yours. PacBio does not collect or store your sequencing data, and SMRT Link Cloud connects directly to your own secure storage. PacBio staff can only access your…
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