Workflow Step: Library preparation
Technical overview — HMW DNA sample preparation for PacBio long-read sequencing using Nanobind PanDNA and SRE kits
I’d like to add automation to my workflow, which automation solution is best for me?
Please use our automation workflow selector tool to determine which of our PacBio Compatible automation solutions are best for your application.
Technical overview — Kinnex library preparation for full-length 16S rRNA sequencing
Technical overview — Kinnex kits for single-cell RNA and full-length RNA and 16S rRNA sequencing
Do my primers need to be 5′ phosphorylated for amplicon sequencing?
No. Your primers do not need to be 5′ phosphorylated for amplicon sequencing.
What are my options for barcoding samples?
You can find all the resources for barcoding all types of samples on the multiplexing page
Which protocol do I need to follow to multiplex microbial libraries for WGS?
You can use the HiFi plex prep kit 96 and this protocol for multiplex microbial WGS and shotgun metagenomics applications
Can I multiplex more than 384 amplicon samples?
Yes. With PacBio barcodes, you can create thousands of asymmetrical combinations for high-throughput multiplexing. While not all combinations have been formally validated, many customers have successfully implemented expanded multiplexing strategies….