Library Preparation Archives

This technical training presentation provides an overview of multiplexed arrays sequencing (MAS-Seq) library preparation using the using the MAS-Seq for 10x Single Cell 3’ kit. Sequencing preparation workflow details, example sequencing performance data, and single-cell MAS-Seq data analysis recommendations are also presented.

November 16, 2022 |

Procedure & checklist – Preparing MAS-Seq libraries using MAS-Seq for 10x 3′ concatenation kit

This procedure describes the workflow for constructing single-cell MAS-Seq (Multiplexed Arrays Sequencing) libraries from 10x Chromium 3’ cDNA using the MAS-Seq for 10x Single Cell 3’ kit for library prep and sequencing on PacBio systems.

November 7, 2022 |

Technical overview – Multiplexed amplicon library preparation using SMRTbell prep kit 3.0

October 20, 2022 |

Insert MAS-Seq for 10x 3′ concatenation kit

Product insert for MAS-Seq for 10x single cell 3′ concatenation kit (PN 102-407-900)

Workflow Step: Library Preparation

Insert – Onso indexed adapter plate

Insert – SMRTbell size selection wash

SDS SMRTbell size selection wash

Quick Reference Card – Commercial workflow product shelf life and storage recommendations

Insert – Onso DNA library prep kit

Insert – Onso fragmentation DNA library prep kit

Insert – Onso library amp kit

Insert – Onso blocker oligos

Insert – Onso indexed library control

Insert – Onso library conversion kit

Insert – Onso library quant kit

Technical overview – MAS-Seq library preparation using the MAS-Seq for 10x Single Cell 3’ kit

Procedure & checklist – Preparing MAS-Seq libraries using MAS-Seq for 10x 3′ concatenation kit

Technical overview – Multiplexed amplicon library preparation using SMRTbell prep kit 3.0

Insert MAS-Seq for 10x 3′ concatenation kit

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