This technical training presentation provides an overview of multiplexed arrays sequencing (MAS-Seq) library preparation using the using the MAS-Seq for 10x Single Cell 3’ kit. Sequencing preparation workflow details, example sequencing performance data, and single-cell MAS-Seq data analysis recommendations are also presented.
This procedure describes the workflow for constructing single-cell MAS-Seq (Multiplexed Arrays Sequencing) libraries from 10x Chromium 3’ cDNA using the MAS-Seq for 10x Single Cell 3’ kit for library prep and sequencing on PacBio systems.
Product insert for MAS-Seq for 10x single cell 3′ concatenation kit (PN 102-407-900)
Safety data sheet for MAS-Seq for 10x Single Cell 3′ kit (PN 102-691-900)
The MAS capture beads kit has streptavidin beads which can be used in combination with a biotynlated probe. It is intended to be used to capture the 3′ end of 10x cDNA molecules.
Safety data sheet for MAS capture beads kit (PN 102-692-000)