Workflow Step: Primary Analysis
Technical overview — PureTarget repeat expansion panel library preparation using PureTarget kit
Technical overview — Kinnex library preparation for full-length 16S rRNA sequencing
Where can I find an overview of all HiFi applications?
Here is an overview of HiFi applications
Technical overview — Kinnex kits for single-cell RNA and full-length RNA and 16S rRNA sequencing
Should I do PCR or probe-based hybridization for my targeted sequencing project?
Typically, customers with many samples find it cost-effective to design a probe panel. View the Application brief – HiFi target enrichment best practices.
Where can I find more information on specific applications?
Explore our Training resources to access on-demand webinars, e-modules, and other educational materials. You can also visit our Sequencing Methods page to learn more about applications such as whole genome…
What analysis tools are available?
PacBio provides a wide range of analysis options to support your research. SMRT Link includes built-in analysis pipelines for many common applications, offering an easy-to-use, integrated environment. For advanced or…
Can I use my short-read sequencing software to analyze long-read sequencing data?
Some short-read analysis tools can be used with long-read data, especially if they include options optimized for longer read lengths. However, most short-read tools are built around assumptions that differ…
What types of genomic variants can be detected from PacBio HiFi data?
PacBio HiFi reads can detect single-nucleotide variants (SNVs), small indels (<20 bp in length) and structural variants (SVs), such as insertions, deletions, duplications, inversions, translocations, short tandem repeats (STRs), and…
Can I use PacBio sequencing systems for targeted sequencing?
Yes, we offer PureTarget panels for PCR free targeted sequencing, hybrid capture workflows with our partners, and amplicon sequencing. Visit the targeted sequencing page for further information.