What is SPRQ chemistry?

SPRQ (pronounced “spark”) is PacBio’s newest HiFi chemistry, designed to make sequencing more efficient and expand HiFi sequencing to more sample types and applications. It requires only 500 ng of native DNA, opening up new possibilities for challenging or precious samples. SPRQ also helps reduce genome costs by increasing HiFi yields per SMRT Cell by 33%, enabling sequencing of two human genomes at 20X coverage per SMRT Cell. In addition, SPRQ supports deeper multiomic insights into chromatin architecture with Fiber-seq, featuring on-instrument 5mC and 6mA calling for greater precision in methylation detection.