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March 19, 2026

What are the key steps in HiFi library preparation and sequencing?

HiFi sequencing offers a streamlined, end-to-end workflow—from sample to analysis. 

Start with high-molecular-weight DNA extraction using Nanobind DNA kits, delivering quality DNA from a wide range of sample types in about two hours. DNA is then converted into SMRTbell libraries using manual or automation-friendly prep kits. 

Libraries are loaded onto Vega or Revio systems, with simple run setup in SMRT Link software. 

After sequencing, advanced algorithms—including Google Health DeepConsensus accelerated by NVIDIA GPUs—process data onboard to generate highly accurate HiFi reads. Outputs are delivered in standardized BAM format, ready for genome assembly, variant detection, phasing, and other downstream analyses. 

This unified workflow reduces complexity and accelerates time to insight. 

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