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Monday, August 30, 2021

Bioinformatics Workflows for High Quality Genome Assembly in Plants and Animals

The Sequel IIe System generates highly accurate long reads (HiFi reads) from genes or regions of interest ranging in size from several hundred base pairs to 20 kb with >99.9% single-molecule read accuracy. HiFi sequencing is the tool of choice to affordably generate contiguous, complete, and correct de novo genome assemblies of even the most complex plant genomes. This webinar will provide an overview of the Maize B73 genome assembly from HiFi sequencing through scaffolding. In this webinar Greg Concepcion will: Review the benefits of HiFi sequencing for genome assembly and examples of assembly metrics for plants and animals Demonstrate assembly and scaffolding of a large diploid plant genome Maize B73 (2.2Gb) with HiFi and Hi-C data for scaffolding, including: Algorithm recommendations Pre- & post-processing recommendations…

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Friday, June 4, 2021

Bioinformatics Workflows for Amplicon Sequencing: from HiFi Data to Results

The Sequel IIe System generates highly accurate long reads (HiFi reads) from genes or regions of interest ranging in size from several hundred base pairs to 20 kb with >99.9% single-molecule read accuracy. This allows you to detect all variant types across relevant regions and sequence through low complexity regions like repeat expansions, promoters, and flanking regions of transposable elements. This webinar will provide an overview of multiplexed amplicon dataset analysis in SMRT Link and the command line and downstream analysis options. In this webinar Bas Tolhuis will cover the points below, followed by a Q&A session: Review recommendations for demultiplexing…

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Monday, May 3, 2021

Advancing Pharmacogenomics Research and the Need for Highly Accurate Long-Read Sequencing

The relatively new field of Pharmacogenomics (PGx) explores how a person’s genome affects their response to drugs to develop safe and effective medications tailored to their genetic makeup. Pharmacogenomic testing enables healthcare providers to tailor a patient’s medication and dosage to their genetic characteristics to help improve outcomes and reduce healthcare costs. Join our webinar to learn more.

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Tuesday, February 23, 2021

Unleashing the Power of HiFi Sequencing – How the Sequel IIe System Removes Barriers and Empowers Life Scientists

With their unique combination of high accuracy and long read lengths, HiFi reads produce superior results across a range of applications, from creation of reference-quality de novo assemblies, to comprehensive variant detection and full-length transcript sequencing. Join us for this webinar to learn how HiFi reads are empowering leading core labs and service providers, and how the new Sequel IIe System, which directly outputs HiFi reads, is making it easier than ever before to get started with HiFi reads or add capacity.

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Wednesday, January 27, 2021

Long Reads Versus More Reads: Comparing Two Sequencing Tools for Evaluating Live Biotherapeutics as Microbiome Interventions

A longstanding challenge in microbiome research has been achieving the taxonomic resolution needed to generate testable hypotheses about how the gut microbiome impacts human health and disease, and to assess the mechanism and efficacy of specific microbiome interventions. Short read whole genome shotgun metagenomic sequencing provides high taxonomic resolution plus functional information but is expensive for large cohort studies. Because of its lower cost, short-read sequencing of the bacterial 16S rRNA gene has become the standard for microbiome profiling, despite its limitations. Recently, HiFi Sequencing with PacBio has offered an alternative to both short-read shotgun metagenomics and partial 16S gene…

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Wednesday, November 11, 2020

University of Pittsburgh Virtual Seminar

As the foundation for advanced scientific discoveries, sequencing data must be accurate and complete. With PacBio highly accurate long reads, known as HiFi reads, scientists no longer need to compromise between long read lengths and high data accuracy. In this webinar, we will illustrate through real-life case studies using HiFi applications to gain comprehensive views of your studied genomes and transcriptomes regardless of their size and complexity.

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Wednesday, September 2, 2020

UC System Virtual Seminar

Get to know the HiFi difference! Join local researchers, and scientists from PacBio, as they update you on the latest SMRT Sequencing applications and capabilities.

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