Quality Statement

Pacific Biosciences is committed to providing high-quality products that meet customer expectations and comply with regulations. We will achieve these goals by adhering to and maintaining an effective quality-management system designed to ensure product quality, performance, and safety.


Image Use Agreement

By downloading, copying, or making any use of the images located on this website (“Site”) you acknowledge that you have read and understand, and agree to, the terms of this Image Usage Agreement, as well as the terms provided on the Legal Notices webpage, which together govern your use of the images as provided below. If you do not agree to such terms, do not download, copy or use the images in any way, unless you have written permission signed by an authorized Pacific Biosciences representative.

Subject to the terms of this Agreement and the terms provided on the Legal Notices webpage (to the extent they do not conflict with the terms of this Agreement), you may use the images on the Site solely for (a) editorial use by press and/or industry analysts, (b) in connection with a normal, peer-reviewed, scientific publication, book or presentation, or the like. You may not alter or modify any image, in whole or in part, for any reason. You may not use any image in a manner that misrepresents the associated Pacific Biosciences product, service or technology or any associated characteristics, data, or properties thereof. You also may not use any image in a manner that denotes some representation or warranty (express, implied or statutory) from Pacific Biosciences of the product, service or technology. The rights granted by this Agreement are personal to you and are not transferable by you to another party.

You, and not Pacific Biosciences, are responsible for your use of the images. You acknowledge and agree that any misuse of the images or breach of this Agreement will cause Pacific Biosciences irreparable harm. Pacific Biosciences is either an owner or licensee of the image, and not an agent for the owner. You agree to give Pacific Biosciences a credit line as follows: "Courtesy of Pacific Biosciences of California, Inc., Menlo Park, CA, USA" and also include any other credits or acknowledgments noted by Pacific Biosciences. You must include any copyright notice originally included with the images on all copies.


You agree that Pacific Biosciences may terminate your access to and use of the images located on the PacificBiosciences.com website at any time and without prior notice, if it considers you to have violated any of the terms of this Image Use Agreement. You agree to indemnify, defend and hold harmless Pacific Biosciences, its officers, directors, employees, agents, licensors, suppliers and any third party information providers to the Site from and against all losses, expenses, damages and costs, including reasonable attorneys' fees, resulting from any violation by you of the terms of this Image Use Agreement or Pacific Biosciences' termination of your access to or use of the Site. Termination will not affect Pacific Biosciences' rights or your obligations which accrued before the termination.

I have read and understand, and agree to, the Image Usage Agreement.

I disagree and would like to return to the Pacific Biosciences home page.

Pacific Biosciences

German Shepherd Genome Project Fetches Popular Vote

Wednesday, April 17, 2019

German Shepherd

Maya and her partner, Sgt. Nic Banuelos, of the UWPD K9 unit

First there was Shadow, the poodle owned by gene-entrepreneur Craig Venter. Then there was Tasha, a female Boxer. Will the next de-coded dog be Maya, a German Shepherd Dog that helps police the campus at the University of Wisconsin-Madison?

Maya has been basking in social media celebrity alongside her human companion Sgt. Nic Banuelos, PhD students Lauren Baker and Emily Binversie, technician Jorden Gruel, veterinary surgeon-scientists Susannah Sample and Peter Muir, and Peter’s woven likeness, after winning the 2019 Plant and Animal SMRT Grant, co-sponsored by Histogenetics.

We caught up with the Comparative Genetics Research Laboratory to find out more about their project, which garnered more than 3,000 votes in a close online video competition amongst six finalists from institutions around the world.

A dog is not a dog is not a dog

When the genome assembly of Tasha the Boxer was released in 2005, little information was available about genomic structural variation in a domesticated species that has breed structure.  At the time, a general consensus in the canine genetic community was well described by Kerstin Lindblad-Toh of the Broad Institute when she stated “A dog is a dog in a genomic sense.”

Not necessarily. As Binversie points out in the UW-Madison team’s crowd-pleaser video, the differences between Ms. Priscella the Papillon and Luna the Great Dane, are extensive, even though they are the same species. The 250+ breeds of dogs display variation in a wide range of traits. There are giant breeds and toy breeds; curly coats and straight coats; short snouts and long snouts; short legs and long legs.

Different breeds also have different disease risks. More than 350 inherited diseases have been described in domestic dogs, nearly half of which predominate in a single breed or a small group of breeds.

Despite this phenotypic variation, there is still only one canine reference genome. It was updated with additional transcriptome data in 2014, but according to Sample and Muir, it’s just not cutting it anymore. The extent of breed-related genomic structural variation remains an important research question.

The winning team: Emily Binversie, Peter Muir, Susannah Sample, Lauren Baker, Jordan Gruel

“Sequencing technologies are evolving rapidly, and they are refining the approaches by which research questions should be addressed,” Muir said. “Maybe, over time, we will be able to focus more on using de novo assembly for genome analysis. Until then, it will be important to have high-quality reference genomes.”

PacBio SMRT sequencing will finally enable Sample to delve deeply into the structural variation that exists between breeds, so that she can better understand their unique traits and diseases.

Among those diseases is fibrotic myopathy, a rare disorder that causes hind limb lameness in the German Shepherd Dog. The condition is especially crippling for service dogs who work with police and military officers, and there are no successful treatment options.

“The selective breeding that occurs creates fascinating genomic architecture specific to each breed. Understanding that further is going to be quite important,” Sample said.

Dog’s best friend

Dogs will not be the only ones to benefit from the project, Muir said.

Since dogs were first domesticated more than 10,000 years ago, they have lived closely alongside humans, and have shared many of the same pathological, dietary and environmental conditions. Understanding their genetic evolution could shed light on ours as well.

Dog diseases are also important models for human disease, Muir said.

“This is a positive step for veterinary medicine in general, and for animal and human One Health studies,” he added.  

The UW-Madison team is currently selecting a candidate dog to sequence. They want a local companion animal or working dog whose health history is well documented, including orthopedic and neurological status. They also intend to follow the health of the dog over time, and conduct additional post-mortem studies for an even richer dataset, possible years into the future when the dog passes away.

Video made the Twitter stars

The “Shroud of Muir”

The team’s first experience with a multimedia grant application was a fun yet frantic one, involving some mass emails to users of the UW–Madison School of Veterinary Medicine to avoid panic when the police K9 unit (and its siren) showed up for the video shoot.

The publicity, which included a brief spot on local television, provided a great opportunity for some community education.

Although Binversie said she hasn’t been asked for any autographs yet, traffic to the group’s Facebook page skyrocketed, and owners of all breeds have been in touch to offer support — and samples.

As for the “Shroud of Muir,” the carpet was given as a gift from a Turkish visiting professor. Instead of being used as a mat for frustrated students to wipe their feet on, as Sample first anticipated, it has a place of honor in the computer laboratory.  


Subscribe for blog updates: