Dr. Olga Vinnere Pettersson, Uppsala Genome Center (Uppsala University), presents best practices for qualifying genomic DNA from a variety of sources to be suitable for Single Molecule, Real-Time Sequencing. Factors that affect single molecule sequencing and recommendations for extracting high-quality genomic DNA will be described. (requires file download to view)
In this webinar, the presenters describe a targeted sequencing workflow that combines Roche NimbleGen's SeqCap EZ enrichment technology with PacBio' SMRT Sequencing to provide a more comprehensive view of variants and haplotype information over multi-kilobase, contiguous regions. They demonstrate that 6 kb fragments can also be utilized to enrich for long fragments that extend beyond the targeted capture site and well into (and often across) the adjacent intronic regions. When combined with SMRT Sequencing, multi-kilobase genomic regions can be phased and variants, including complex structural variants, can be detected in exons, introns and intergenic regions.
In this webinar, Elizabeth Tseng from PacBio demonstrates how to run the Iso-Seq bioinformatics software pipeline that is part of PacBio's SMRTAnalysis software suite. Both the web portal interface (SMRT Portal) and the command line version will be introduced. In addition, she reviews the community version of Iso-Seq (pbtranscript-tofu) and other community tools to perform additional analyses.
In this webinar, Tyson Clark from PacBio discusses the recent Iso-Seq template preparation protocol updates for creating full-length cDNAs and discusses considerations for experimental design.
This seminar features great hands-on information and best practices for analyzing SMRT Sequencing data for eukaryotic genome assembly. Michael Schatz provides an overview of the assembly tools, provides recommendations for when to use each one, and discusses the challenges of short-read assemblies. James Gurtowski gives an in-depth overview of hybrid assemblies methods, where short read data are used used to correct errors in longer reads. Finally, Sergey Koren presents on chromosome-scale assembly, including the MinHash Alignment Process (MHAP) he developed to dramatically reduce the computational processing power required for genome assemblies.
PacBio scientists Kristi Kim and Michael Weiand present on high-throughput solutions for sample preparation including 10 kb template preparation, high-throughput PacBio RS II sample prep kits, and compatible automated liquid handling platforms.
This animation depicts a process by which single molecule SMRTbell templates are loaded in the Zero Mode Waveguides (ZMWs) of the PacBio RS II sequencing system using the automated MagBead Station.