Asset Tag: Targeted sequencing,
June 24, 2022 |
Quick Reference Card — Loading and Pre-Extension Recommendations for the Sequel II and IIe Systems
May 19, 2022 |
Procedure & checklist — Amplification of bacterial full-length 16S rRNA gene with barcoded primers
May 16, 2022 | Gene therapy + editing
Application brochure — AAV Sequencing with HiFi Reads
With Single Molecule, Real-Time (SMRT®) sequencing on the Sequel® IIe systems, you can sequence AAV
genome populations to identify truncation, mutation, and host integration events. The AAV workflow from
PacBio® accommodates both scAAV and ssAAV constructs with easy on-instrument HiFi read generation
November 15, 2021 | Products, procedures + protocols
Brochure — SMRT Sequencing: Delivering highly accurate long reads to drive discovery in life science
Learn how Single Molecule, Real-Time (SMRT) Sequencing and the Sequel IIe System and will accelerate your research by delivering highly accurate long reads to provide the most comprehensive view of genomes, transcriptomes and epigenomes.
November 15, 2021 |
Application brochure — What can you do with one SMRT Cell?
With PacBio Single Molecule, Real-Time (SMRT) Sequencing on the Sequel IIe System you can characterize whole genomes and transcriptomes with just one SMRT Cell. Explore our applications and pricing to get your sequencing project started.
November 15, 2021 |
Procedure & Checklist — Preparing SMRTbell Libraries using PacBio Barcoded Overhang Adapters for Multiplexing Amplicons
November 2, 2021 |
HiFi Sequencing: See What You’ve Been Missing
PacBio Vice President of Segment Marketing, Dr. Jennifer Stone, demonstrates how HiFi sequencing is changing the game in human genetics by sharing some of the exciting milestones and seminal publications…
October 29, 2021 |
Targeting Clinically Significant Dark Regions of the Human Genome with High-Accuracy, Long-Read Sequencing
There are many clinically important genes in “dark” regions of the human genome. These regions are characterized as dark due to a paucity of NGS coverage as a result of short-read sequencing or mapping difficulties. Low NGS sequencing yield can arise in these regions due to the presence of various repeat elements or biased base composition while inaccurate mapping can result from segmental duplications. Long-read sequencing coupled with an optimized, robust enrichment method has the potential to illuminate these dark regions.
October 29, 2021 |
Resolving Complex Pathogenic Alleles using HiFi Long-Range Amplicon Data and a New Clustering Algorithm
Many genetic diseases are mapped to structurally complex loci. These regions contain highly similar paralogous alleles (>99% identity) that span kilobases within the human genome. Comprehensive screening for pathogenic variants is incomplete and labor intensive using short-reads or optical mapping. In contrast, long-range amplification and PacBio HiFi sequencing fully and directly resolve and phase a wide range of pathogenic variants without inference. To capitalize on the accuracy of HiFi data we designed a new amplicon analysis tool, pbAA. pbAA can rapidly deconvolve a mixture of haplotypes, enabling precise diplotyping, and disease allele classification.
October 28, 2021 |
Resolving Highly Diverse HLA and CYP2D6 Alleles Using HiFi Sequencing for Long-Range Amplicon Data with a New Clustering Algorithm
Targeted amplification of difficult pharmacogenetic loci with PacBio HiFi reads can resolve complex alleles in a single direct assay without imputation.
October 28, 2021 |
Long-Read Amplicon Sequencing of the Polymorphic CYP2D6 Locus
With PacBio Single Molecule, Real-Time (SMRT) Sequencing on the Sequel IIe System you can characterize highly polymorphic CYP2D6 locus from 384 or more samples with just one SMRT Cell. Explore our applications and pricing to get your sequencing project started.
October 28, 2021 |
Development and Optimization of a 43 Gene Pharmacogenomic Panel Using Enrichment-Based Capture and PacBio HiFi Sequencing
We developed a novel method to comprehensively interrogate a panel of 43 pharmacogenomic genes using an enrichment-based capture strategy (IDT) coupled with PacBio highly accurate (HiFi) Sequencing.
October 28, 2021 |
Using HiFi Reads for Improved and Accurate Haplotyping and Phasing of Pharmacogenomic Alleles
Pharmacogenomics (PGx) utilizes genomic information to assess an individual’s response to certain medications and can be used to predict adverse drug reactions or decreased efficacy. While numerous assays and genetic…