Asset Tag: Sequel II System
Application brochure — AAV Sequencing with HiFi Reads
With Single Molecule, Real-Time (SMRT®) sequencing on the Sequel® IIe systems, you can sequence AAV
genome populations to identify truncation, mutation, and host integration events. The AAV workflow from
PacBio® accommodates both scAAV and ssAAV constructs with easy on-instrument HiFi read generation
Brochure — SMRT Sequencing: Delivering highly accurate long reads to drive discovery in life science
Learn how Single Molecule, Real-Time (SMRT) Sequencing and the Sequel IIe System and will accelerate your research by delivering highly accurate long reads to provide the most comprehensive view of genomes, transcriptomes and epigenomes.
Application brief — Microbial whole genome sequencing
With Single Molecule, Real-Time (SMRT) Sequencing and the Sequel Systems, you can affordably assemble reference-quality microbial genomes that are >99.999% (Q50) accurate.
Application brochure — What can you do with one SMRT Cell?
With PacBio Single Molecule, Real-Time (SMRT) Sequencing on the Sequel IIe System you can characterize whole genomes and transcriptomes with just one SMRT Cell. Explore our applications and pricing to get your sequencing project started.
HiFiViral SARS-CoV-2: A Kitted Solution for Genome Surveillance that is Robust Across Sample Input Quantities and New Variants
The COVID-19 pandemic continues to be a major global epidemiological challenge with the ongoing emergence of new strain lineages that are more contagious, more virulent, drug resistant and in some cases evade vaccine-induced immunity. In response, the HiFiViral SARS-CoV-2 kit (PacBio; Menlo Park, California) was developed as a scalable solution for the Sequel II and Sequel IIe systems. The HiFiViral SARS-CoV-2 is an easy to perform solution for surveillance of variants to support pandemic response by public health. With 80% of samples yielding complete genome coverage in a 96-plex run, the combination of long read lengths and a differentiated probe design provides highly accurate results and robust genome coverage across a range of Ct values.
Resolving Highly Diverse HLA and CYP2D6 Alleles Using HiFi Sequencing for Long-Range Amplicon Data with a New Clustering Algorithm
Targeted amplification of difficult pharmacogenetic loci with PacBio HiFi reads can resolve complex alleles in a single direct assay without imputation.
Long-Read Amplicon Sequencing of the Polymorphic CYP2D6 Locus
With PacBio Single Molecule, Real-Time (SMRT) Sequencing on the Sequel IIe System you can characterize highly polymorphic CYP2D6 locus from 384 or more samples with just one SMRT Cell. Explore our applications and pricing to get your sequencing project started.
Simplified and Robust Library Construction for High-Throughput HiFi Sequencing for Human Variant Detection
New workflow for preparing SMRTbell libraries from <4ug gDNA input using AMPure PB bead purification. This workflow requires samples with high molecular weight gDNA with minimal presence of <5kb fragments.
Development and Optimization of a 43 Gene Pharmacogenomic Panel Using Enrichment-Based Capture and PacBio HiFi Sequencing
We developed a novel method to comprehensively interrogate a panel of 43 pharmacogenomic genes using an enrichment-based capture strategy (IDT) coupled with PacBio highly accurate (HiFi) Sequencing.
Towards Isoform Resolution Single-Cell Transcriptomics for Clinical Applications Using Highly Accurate Long-Read Sequencing
Using concatenation we increase single-cell Iso-Seq (scIso-Seq) throughput to ~8 million de-concatenated full-length molecules per SMRT Cell 8M. We show that the scIso-Seq method captures full-length isoform information at the single-cell level.
Technical note — Preparing DNA for PacBio HiFi sequencing – Extraction and quality control
Single Molecule, Real-Time (SMRT) Sequencing uses the natural process of DNA replication to sequence long fragments of native DNA in order to produce highly accurate long reads, or HiFi reads. As such, starting with high-quality, high molecular weight (HMW) genomic DNA (gDNA) will result in longer libraries and better performance during sequencing. This technical note is intended to give recommendations, tips and tricks for the extraction of DNA, as well as assessing and preserving the quality and size of your DNA sample to be used for HiFi sequencing.
Brochure — Sequel II system: Delivering highly accurate long reads
The Sequel II System, powered by Single Molecule, Real Time (SMRT) Technology, delivers highly accurate long reads for a comprehensive view of genomes, transcriptomes and epigenomes.