Genome sequence of Pseudomonas koreensis CRS05-R5, an antagonistic bacterium isolated from rice paddy field.
Pseudomonas koreensis, a new nominated Gram-negative bacterium was first reported and isolated from Korean agricultural soil (Kwon et al., 2003). CRS05-R5 (first reported as Pseudomonas sp.), which showed biocontrol ability against Sitophilus oryzae and Acidovorax avenae subsp. avenae (Liu et al., 2014), was first isolated from the rice rhizosphere in Heilongjiang province and reported in 2003 (Xie et al., 2003). Except for that, this species has been reported to produce the biosurfactant, which has biocontrol ability against Phytophthora infestans and Pythium ultimum (Hultberg et al., 2010a,b). These interesting features raise our attention on CRS05-R5. Recently, we sequenced the 16S rRNA sequence from CRS05-R5 and built the phylogenetic tree (Figure S1). Based on that, we confirmed that CRS05-R5 should be classified as P. koreensis. However, only one genome was sequenced (D26) and no detailed analysis was performed on this species. In this case, we did whole-genome sequencing on CRS05-R5, and tried to reveal the possible mechanism behind its antagonistic ability.