The bacteria living on and within us can impact health, disease, and even our behavior, but there is still much to learn about the breadth of their effects. The torrent of new discoveries unleashed by high-throughput sequencing has captured the imagination of scientists and the public alike. Scientists at Second Genome are hoping to apply these insights to improve human health, leveraging their bioinformatics expertise to mine bacterial communities for potential therapeutics. Recently they teamed up with scientists at PacBio to explore how long-read sequencing might supplement their short-read-based pipeline for gene discovery, using an environmental sample as a test…
Many scientists are using PacBio Single Molecule, Real-Time (SMRT) Sequencing to explore the genomes and transcriptomes of a wide variety of marine species and ecosystems. These studies are already adding to our understanding of how marine species adapt and evolve, contributing to conservation efforts, and informing how we can optimize food production through efficient aquaculture.
Highly accurate long reads – HiFi reads – with single-molecule resolution make Single Molecule, Real-Time (SMRT) Sequencing ideal for full-length 16S rRNA sequencing, shotgun metagenomic profiling, and metagenome assembly.
With PacBio Single Molecule, Real-Time (SMRT) Sequencing on the Sequel IIe System you can characterize whole genomes and transcriptomes with just one SMRT Cell. Explore our applications and pricing to get your sequencing project started.
Learn how highly accurate long-read sequencing from the Sequel IIe Systems delivers data you can trust for advanced biological insights across a range of applications.
Discover the benefits of HiFi reads and learn how highly accurate long-read sequencing provides a single technology solution across a range of applications.
Learn how Single Molecule, Real-Time (SMRT) Sequencing and the Sequel IIe System and will accelerate your research by delivering highly accurate long reads to provide the most comprehensive view of genomes, transcriptomes and epigenomes.
Shane Brubaker from renewable oil manufacturer Solazyme reports using the PacBio system to sequence the genome of a GC-rich strain of algae that couldn’t be fully assembled with short-read sequence data. He notes that CCS reads exceed Sanger quality at significantly lower cost.
Lizzie Wilbanks formerly from UC Davis, discusses how longs read from SMRT Sequencing allow accurate assembly of members from the complex pink berry salt marsh community.
Tim Smith from the USDA’s Agricultural Research Service talks about using long-read sequencing to redo assemblies for cattle, swine, sheep, and goat. Long-read assemblies correct misassemblies and other problems from older genome projects. Smith says SMRT Sequencing has also made possible isoform sequencing as well as microbiome characterization.
PacBio scientist Cheryl Heiner describes new low-input protocols for SMRT Sequencing library construction. With these revised methods, 2 kb libraries can be generated from as little as 10 ng of DNA, while 10 kb libraries require only 100 ng of sample.
In this AGBT poster, Cheryl Heiner from PacBio describes results from a variety of experiments optimizing a protocol for full-length 16S amplification for SMRT Sequencing.