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Sequel System 6.0 Release

Don’t compromise read length for accuracy when you can have both

What's new in the release

The Sequel System 6.0 release features major improvements to system performance through our new 3.0 consumables, SMRT Cell 1M v3, Sequel ICS v6.0, and SMRT Link v6.0. Combined, these enhancements enable:

  • Up to 500,000 long (1–20 kb), single-molecule reads with high fidelity (>99% accuracy) for amplicon and RNA sequencing projects
  • Up to 20 Gb per SMRT Cell 1M with average read lengths up to 30 kb and achieve high consensus accuracies (>99.999%) for whole genome sequencing projects

Release Performance

 

Long Read Lengths
(Libraries >20 kb)

Half of data in reads: >45 kb
Data per SMRT Cell: Up to 20 Gb

Long Read Lengths
(Libraries <20 kb)

Half of data in reads: >190 kb
Data per SMRT Cell: Up to 50 Gb

Read length data from a 35 kb  (left) and 15 kb (right) size-selected human library using the SMRTbell Express Template Prep Kit on a Sequel System*.

*Read lengths, reads/data per SMRT Cell and other performance results vary based on sample quality/type and insert size.

 

High-Fidelity, Long Reads

Estimated number of high-fidelity, long reads (Q20 or Q30) per Sequel SMRT Cell  based on insert size and movie collection time.

High Consensus Accuracy

Consensus accuracy is a function of coverage and chemistry. The data above is based on a bacterial genome run on the Sequel System. Single-molecule accuracy has similar coverage requirements.

 

Learn more about SMRT Sequencing


Spotlight on Applications

Whole Genome Sequencing

  • Improved consensus accuracy for complex genomes
  • Push button microbial assembly
  • Characterize performance of 4mC, 6mA base modification detection in microbial genomes
Structural Variant Calling

  • Now calling inversions, translocations, and small indels >20-49 bp
  • Faster runtime and more scalable workflow
  • Example dataset
RNA Sequencing

Introducing the Iso-Seq 3 method featuring:

  • Faster runtime, improved stability and scalability
  • Unified support for demultiplexing
Targeted Sequencing

  • Generate up to 500,000 high quality, Q20 reads, depending upon insert size
  • Improved read lengths for amplicons >5 kb resulting in significant increase in Q20 reads

New SMRT Sequencing 3.0 Consumables

Product NameProduct Number
Sequel Binding and Internal Control Kit 3.0101-626-600
Sequel Sequencing Kit 3.0 Bundle (5 packs, 4 rxn); (5 packs, 8 rxn)101-642-400; 101-642-300
Sequel Sequencing Kit 3.0 (4 rxn); (8 rxn)101-597-900; 101-597-800
SMRT Cell 1M v3 Tray101-531-000
SMRT Cell 1M v3 LR Tray101-531-001

 

See a complete list of all SMRT Consumables and Documentation

 

 

 

 


 

SMRT Link v6.0 Software Download

    Event

    PAG XXVII

    January 12, 2019-January 16, 2019

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