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Sequel II System - Coming Soon!

PacBio is excited to announce the upcoming launch of the Sequel II System

Delivering highly accurate long reads

The PacBio Sequel II System provides the advantages of SMRT Sequencing with ~8-times the data making it more affordable for all scientists to drive discovery with comprehensive views of genomes and transcriptomes.

 

 
 
 

The HiFi Difference

 

You no longer have to choose between high accuracy and long read lengths. With HiFi reads, powered by the CCS method, you get highly accurate long-read sequencing data you can trust.

Explore how HiFi reads are enabling calling of all variant types in the human genome.

Learn more

The Sequel II System – The Next Evolution of SMRT Sequencing

Watch Marty Badgett present on the Sequel II System at AGBT 2019

Pre-Release System Performance

Early example data from genomic libraries generated using the continuous long read (CLR) and HiFi read modes of sequencing on the Sequel II System.

CLR Library

Throughput: 99 Gb

Half of based in reads >33,000

Data shown above from a 35 kb size-selected human library using the SMRTbell Template Prep Kit on a Sequel II System* (0.9 Chemistry, Sequel II System Software v6.1, 10-hour movie).

HiFi Library

Number of >Q20 (99%) Bases: 21 Gb

Number of >Q20 Reads: 1,800,000

Data shown above from a 12 kb size-selected human library using the SMRTbell Template Prep Kit on a Sequel II System* (0.9 Chemistry, Sequel II System Software v6.1, 30-hour movie).

*Read lengths, reads/data per SMRT Cell 8M and other sequencing performance results may vary based on sample quality/type and insert size.

High Consensus Accuracy

Achieves >99.999% (QV50)

Consensus accuracy is a function of coverage and chemistry. The data above is based on a bacterial genome run on the Sequel II System (0.9 Chemistry, Sequel II System Software v6.1). HiFi read accuracy has similar coverage requirements.

Uniform Coverage

Even coverage across GC content

Mean coverage per GC window across a human sample run on the Sequel II System (0.9 Chemistry, Sequel II System Software v6.1)

 


 

Spotlight on Applications

Whole Genome Sequencing

  • Generate high-quality de novo assemblies
Variant Detection

RNA Sequencing

  • Read full-length transcripts to characterize isoform diversity

Want to stay up-to-date on the Sequel II System release?

Previous Release Infomation

Sequel System 6.0 Release