Blog
This blog features voices from PacBio — and our partners and colleagues — discussing the latest research, publications, and updates about HiFi sequencing.
Blog
Now Available: Ultra-Low DNA Input Workflow for SMRT Sequencing
It’s one of the questions we hear most often from scientists working with small organisms: Is it possible to generate truly high-quality, long-read data from minuscule amounts of DNA? With…
High Quality HiFi Assemblers Open Up a Wide New World of Genomics Possibilities
With PacBio HiFi sequencing data now readily available for organisms of any size, many exciting results have been published featuring new de novo assembly methods optimized for highly accurate long…
Colombian SMRT Grant winners hope HiFi sequencing will help save critically endangered toads
Meet the ‘happy toad’ Atelopus laetissimus, a harlequin toad found on the slopes of the Sierra Nevada de Santa Marta mountains of Colombia. This toad is brightly colored, with an…
Pangenome of Soybean Generated to Capture Genomic Diversity
It has recently become apparent how important it is to sequence more than one individual to characterize the genomic variation within a species. This makes sense if you consider that…
A TAL Tale: PacBio Sequencing Helps Unravel Mechanisms of Plant Infection
How do bacteria manipulate plant biology to cause blight and rot? Why are some pathogen strains more virulent than others? How can we engineer resistant staple food crops? These are…
In Alabama, Scientists Use HiFi Data to Uncover the Genetic Cause of Rare Neurodevelopmental Disorders
UPDATE: This paper has now been published in HGG Advances from Cell Press In an exciting new preprint, scientists from the HudsonAlpha Institute for Biotechnology and the University of…
In precisionFDA Challenge, PacBio HiFi Reads Outperform Both Short Reads and Noisy Long Reads
In the recent precisionFDA Truth Challenge V2, which evaluated methods for variant calling in human genomes, approaches that use PacBio HiFi reads delivered the highest precision and recall in all…
“Murder Hornet” Genome Rapidly Assembled by USDA as Part of Real-Time Invasive Species Response Initiative
With a nickname like “murder hornet,” it’s no wonder the two-inch long Asian giant hornet (Vespa mandarinia) has caused a stir amongst those terrified of insect invasions. The wasps —…
A HiFi View: Sequencing the Gut Microbiome with Highly Accurate Long Reads
Whether you are seeking to characterize microbial diversity in the gut, or distinguish between pathogenic and commensal bacteria on the skin, full-length 16S rRNA sequencing using PacBio systems is a…
Sequencing 101: what’s the value of sequencing full-length RNA transcripts?
The study of genomics has revolutionized our understanding of science, but the field of transcriptomics grew with the need to explore the functional impacts of genetic variation. While different tissues…
Review: How Long-Read Sequencing Is Revealing Unseen Genomic Variation
“We are now embarking on an era where all genetic variation in an individual will be completely discovered,” write Glennis Logsdon (@glennis_logsdon), Mitchell Vollger (@mrvollger), and Evan Eichler in a recent…
New Isoform Phasing Technique Traces Parental-Progeny Differences in Maize
It’s not unusual for progeny to outperform their parents, and it’s often the goal in plant breeding. But tracing the molecular basis of such heterosis can be difficult, especially…
Sequencing 101: from DNA to discovery — the steps of SMRT sequencing
Starting a sequencing project can be daunting. First of all, there are several types of sequencing technologies, each based on unique processes. At PacBio, we use a technology called Single…
Don’t Sweat The Small Stuff: Low DNA Input Workflow Enables Sequencing of the Smallest Species
Tackling larger and larger genomes has been an attractive pursuit for many scientists as sequencing technologies improve at rapid rates. But what about the other end of the spectrum —…
Webinar: Increasing Solve Rates for Rare and Mendelian Diseases with Long-Read Sequencing
It’s well known that finding the genetic cause of rare diseases can be complex — that’s why so many remain unsolved. But researchers are beginning to get a grasp on…