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Understand the Evolving Pandemic with PacBio Sequencing

The spread of new variants has made whole genome sequencing a newly critical part of the global SARS-CoV-2 response. In this new phase of the pandemic, PacBio is committed to supporting government, academic, and commercial labs in their efforts to increase virus surveillance.


Targeted Sequencing for SARS-CoV-2 Surveillance

HiFi reads from the Sequel IIe System provide a reliable, cost effective, high-throughput solution for sequencing SARS-CoV-2 quickly and easily.

 

HiFiViral for SARS-CoV-2 Workflow

 

Library Prep

Amplify and barcode up to 900 SARS-CoV-2 samples for multiplexing in a single library

Sequencing

Use the Sequel II or Sequel IIe Systems to generate >99.9% accurate HiFi reads

Analysis Solutions

Assemble genomes and call all variants with our efficient analytical software tools and standard file formats

 

Protocol: HiFiViral for SARS-CoV-2 Workflow – Multiplexing 1.2 kb Amplicons for Full-Viral Genome Sequencing

 

This protocol describes a workflow for multiplexing 1.2 kb amplicons for full-viral genome sequencing of SARS-CoV-2 samples on the Sequel II or Sequel IIe Systems for genome assembly or variant calling.

Sequences for the 29 pairs of M13-tailed primers are available using this oligo ordering sheet.

 

 

 

 

 

Benefits of this Protocol for SARS-CoV-2 Research

 

Fewer Dropouts

With only 29 amplicons, primers anneal to just 4.5% of the viral genome compared to 17-40% with other assays, lowering the chance that viral mutations will cause dropouts due to primer mismatches

 

Easier Primer Balancing

The use of 1.2 kb amplicons optimizes for even and complete genome coverage by balancing multiplex PCR simplification against the challenge of sample-quality variability

 

Call All Variants

Using >99.9% accurate HiFi reads you can detect both SNPs and indels up to 300 bp in length with only 4-fold coverage

 

 

LabCorp: Geographic and Temporal Mapping of SARS-CoV-2 Pandemic

Watch this video to learn how LabCorp is using HiFi sequencing and the Sequel II System in their population-scale surveillance of SARS-CoV-2 mutants in the United States.

 

Learn More about LabCorp’s Surveillance Efforts

Advantages of HiFi Sequencing on the Sequel IIe System:

 

  • Better data for superior results with lower coverage, from a single technology
  • Less time spent on data processing and analysis for faster answers
  • Cost savings at every step of your sequencing pipeline
  • Reliable high-throughput sequencing for a broad range of applications

 

 

 


PacBio Resources

In the News

First case of South African COVID variant detected in Virginia

Why COVID-19 Variant Might Be ‘Too Much’ for Current Vaccines

Press Releases

Pacific Biosciences Sequel II Systems Deployed to Scale-Up Global Viral Surveillance Initiatives Focused on COVID-19

South Carolina Public Health Officials Detect Nation’s First Known Cases of the COVID-19 Variant Originally Detected in South Africa

SARS-CoV-2 Viral Genome Sequencing Data Presented from Research Using Pacific Biosciences Technology

Pacific Biosciences Powers SARS-CoV-2 Research at Commercial, Academic and Government Labs

Blog Posts

Why Are Long Reads Important for Studying Viral Genomes?

In Battle Against COVID-19 Pandemic, Scientists Turn to PacBio Sequencing

Webinars

Opportunities for using PacBio Long-read Sequencing for COVID-19 Research

Understanding SARS-CoV-2 and Host Immune Response to COVID-19 with PacBio Sequencing

Infographic:

Mapping the interplay of viral and host genomics with HiFi sequencing

Relevant Publications on Viral Sequencing


Connect with a PacBio Scientist

 

If you are working on coronavirus or COVID-19 and would like to learn more about protocols or service providers, please contact us.

 

 


 

Read the PacBio Statement on Our COVID-19 Response

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