April 21, 2020  |  

Complete genome sequence of Euzebya sp. DY32-46, a marine Actinobacteria isolated from the Pacific Ocean

Authors: Xu, Lin and Sun, Cong and Huang, Mengmeng and Wu, Yue-Hong and Yuan, Chang-Qing and Dai, Wen-Hua and Ye, Kaixiong and Han, Bingnan and Xu, Xue-Wei

Euzebya sp. DY32-46 was isolated from seawater collected at the depth of 150?m in the eastern Pacific Ocean. The genome was sequenced and consisted of one chromosome and one plasmid pEDY32-46I with sizes of 5,799,875 and 571,580?bp as well as DNA G?+?C contents of 70.7 and 69.6%, respectively. Genomic annotation showed that fifty biosynthetic gene clusters of secondary metabolites were located in the chromosome of Euzebya sp. DY32-46, indicating that a wide variety of its natural products need to be explored. Besides, dozens of biogeochemically relevant genes found in the genome of Euzebya sp. DY32-46 revealed its ecological roles in marine carbon, nitrogen, phosphorus and sulfur cycles including transporting ammonium, phosphate and methylammonium, cleaving dimethylsulfoniopropionate and phosphate, reducing nitrite, etc. Based on genomic similarity analysis, Euzebya sp. DY32-46 represents a novel genospecies of the genus Euzebya, with average nucleotide identity and digital DNA-DNA hybridization values of 73.1-87.1% and 20.2-32.4% compared with the closely related type strains Euzebya rosea DSW09T and E. tangerina F10T. Comparative genomic analysis revealed that 97.1% of coding sequences were exclusively present in the plasmid pEDY32-46I contributing the speciation of Euzebya sp. DY32-46. This study widens our knowledge about industrial potential as well as ecological roles of the genus Euzebya and provides a great candidate for investigating nascent speciation of marine Actinobacteria.

Journal: Marine genomics
DOI: 10.1016/j.margen.2018.09.008
Year: 2019

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