April 21, 2020  |  

Circular consensus sequencing with long reads.

Authors: Tang, Lei

Long-read sequencing technologies have advantages in genome assembly, structural variant detection and haplotype phasing, but are less suited for single-nucleotide variant (SNV) and insertion/deletion (indel) calling due to the high error rate in comparison with short-read sequencing. Wenger et al., from Pacific Biosciences, optimized the circular consensus sequencing (CCS) protocol to achieve long, high-fidelity reads, in which they selected the SMRTbell library with fractions tightly distributed at 15 kb for high-coverage sequencing.

Journal: Nature methods
DOI: 10.1038/s41592-019-0605-6
Year: 2019

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