Blog
This blog features voices from PacBio — and our partners and colleagues — discussing the latest research, publications, and updates about HiFi sequencing.
Blog
AGBT Day 1 & 2 Highlights: Hello GRCh38 & SMRT Sequencing for Pathogen Screening
AGBT 2014 is off to a roaring start – the opening reception was hastily moved indoors when an impressive thunderstorm joined the party. Wednesday’s kickoff plenary session offered an insightful…
Data Release: ~54x Long-Read Coverage for PacBio-only De Novo Human Genome Assembly
We are pleased to make publicly available a new shotgun sequence dataset of long PacBio® reads from a human DNA sample. We previously released sequence data using Single Molecule, Real-Time…
AGBT 2014 Preview: Long reads, long flight, long days!
We are flying cross-country to Marco Island, Florida, to attend the fifteenth annual Advances in Genome Biology and Technology conference and, as we have done for years now, we are…
At Plant & Animal Genome Workshop, Users Showcase Projects Enabled by SMRT Sequencing
Earlier this month, we hosted a workshop at the International Plant & Animal Genome (PAG) conference in San Diego entitled “A SMRT® Sequencing Approach to Reference Genomes, Annotation, and Haplotyping.”…
PacBio Service Provider DNA Link Sees Soaring Global Demand for SMRT Sequencing
Since its founding in 2000, the service provider team at Korea-based DNA Link has sought to differentiate itself from other facilities by being an early adopter of new technologies. The…
Genome Research Paper: Resolve Complex Genomic Regions for a ‘Fraction of the Cost’ With SMRT Sequencing
A new Genome Research paper describes the application of Single Molecule, Real-Time (SMRT®) Sequencing to resolve repeat-heavy genomic regions in important reference genomes such as human and chimpanzee. In the…
Looking Ahead: The 2014 PacBio Technology Roadmap
By Jonas Korlach, Chief Scientific Officer 2013 was an eventful and exciting year for PacBio. As I described in the 2013 roadmap post a year ago, we have applied numerous…
Data Release: Preliminary de novo Haploid and Diploid Assemblies of Drosophila melanogaster
Model organisms such as yeast, Arabidopsis and Drosophila have been essential to progress in genetic and biomedical research for more than 100 years. Model organisms are the best, fastest, most…
SMRT Sequencing for Plant and Animal Genomes: Learn More at PAG 2014
Many recent studies have demonstrated the use of Single Molecule, Real-Time (SMRT®) Sequencing for larger genomes, from complete reference genomes to de novo discovery of transcript isoforms. These advances include…
Breakpoint Detection in Cancer Structural Variants with PacBio May Yield Patient-Specific Data
A new publication from scientists at the University of California, San Diego, demonstrates the use of Single Molecule, Real-Time (SMRT®) Sequencing to identify structural variation (SV) breakpoints in cancer. “Amplification…
New Publication Characterizes the Complex Methylomes of Helicobacter pylori
A new paper in Nucleic Acids Research describes the genome-wide methylation state of two strains of Helicobacter pylori, using Single Molecule, Real-Time (SMRT®) Sequencing. The paper represents the first comprehensive…
In RNA-seq Study, Long PacBio Reads Allow for Detection of Full-Length and Novel Isoforms
A new paper out in PNAS details the usefulness of long reads for isoform sequencing. “Characterization of the human ESC transcriptome by hybrid sequencing” comes from lead author Kin Fai…
PacBio Partners with Sanger Institute and Public Health England to Finish 3,000 Bacterial Genomes
Sanger’s Genome Campus We are pleased to announce a new collaboration with the Wellcome Trust Sanger Institute and Public Health England to complete the sequences of 3,000 bacterial genome strains…
New Publication Demonstrates Long-Read Sequences Needed to Thoroughly Resolve Short Tandem Repeats
In a new paper reporting a protocol for using short-read sequence data to locate short tandem repeats (STRs), scientists find that long-read sequence information is necessary to resolve regions with…
ASHG Workshop Recordings: Resolving Structural Variation in Human Genomes
We hosted a structural varation workshop at the annual meeting of the American Society of Human Genetics, and were pleased to see that the speakers’ presentations really resonated with attendees…