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Featured Documentation

This Featured Documentation page provides the latest and most frequently used guides, protocols (procedures & checklists), and product information. See Other Documentation for product inserts, safety data sheets (SDS) and more.

February 23, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for non-human mammalian blood NHMB on the Hamilton NIMBUS Presto system

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Extraction, Sample preparation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50-300 kb) DNA from 200 μL of non-human mammalian blood (NHMB) using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
February 23, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for nucleated red blood cells nRBCs on the Hamilton NIMBUS Presto system

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Extraction, Sample preparation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 5 μL of nucleated red blood cells (nRBCs) using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
February 23, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for nucleated red blood cells nRBCs on the KingFisher Apex system

  • Procedure & checklist
  • Sequel II system and Sequel IIe system, Revio system
  • Extraction, Sample preparation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 5 μL of nucleated red blood cells (nRBCs) using the Thermo Fisher KingFisher Apex system. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
June 8, 2023

Guide & overview — Nanobind tissue kit

  • Guide & overview
  • Sequel II system and Sequel IIe system, Revio system
  • Sample preparation

Description

This document describes procedures on how to use the Nanobind tissue kit for extraction of HMW (50 kb – 300+ kb) and UHMW (50 kb – 1+ Mb) DNA from animal tissue samples.
May 25, 2023

Guide & overview — Nanobind CBB kit

  • Guide & overview
  • Sequel II system and Sequel IIe system, Revio system
  • Sample preparation

Description

This document describes procedures on how to use the Nanobind CBB Kit for extraction of HMW (50 kb – 300+ kb) DNA from cultured cells, cultured bacteria, and blood.
January 17, 2023

Overview — Nanobind HMW DNA extraction overview

  • Guide
  • Sequel II system and Sequel IIe system, Revio system
  • Sample preparation

Description

This overview provides links to all standard procedures for extracting HMW DNA by sample type and compatible Nanobind kit.
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