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Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for mammalian cultured cells on KingFisher Duo Prime system

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for mammalian cultured cells on KingFisher Flex system

Guide & overview — Short Read Eliminator (SRE) XS and XL kits

Description

This document describes procedures on how to use the the SRE kit family (SRE XS, SRE XL) for rapid high-pass size selection of high molecular weight (HMW) DNA samples.

Procedure & checklist — Removing short DNA fragments with the Short Read Eliminator (SRE) kit

Description

This procedure describes the workflow for DNA size selection to remove molecules <10 kb using the SRE kit on high molecular weight (HMW) DNA before shearing and library preparation.

Quick Reference Card — Commercial workflow product shelf life and storage recommendations

Description

PacBio® reagent kits are guaranteed a minimum shelf life of 90 days upon customer receipt. For specific kit storage conditions, please see the product packaging and insert.

Overview — Nanobind HMW DNA extraction overview

Description

This overview provides links to all standard procedures for extracting HMW DNA by sample type and compatible Nanobind kit.

Procedure & checklist — Extracting HMW DNA from cultured cells using Nanobind kits

Description

This procedure can be used to extract HMW DNA from cultured cells.

Procedure & checklist — Extracting HMW DNA from fish skeletal muscle using Nanobind kits

Description

This procedure can be used to isolate HMW DNA from fish skeletal muscle.

Procedure & checklist — Extracting HMW DNA from Gram-negative bacteria using Nanobind kits

Description

This procedure can be used to extract HMW DNA from Gram-negative bacteria.

Procedure & checklist — Extracting HMW DNA from Gram-positive bacteria using Nanobind kits

Description

This procedure can be used to extract HMW DNA from Gram-positive bacteria.

Procedure & checklist — Extracting HMW DNA from mammalian whole blood using Nanobind kits

Description

This procedure can be used to extract HMW DNA from mammalian whole blood.

Procedure & checklist — Extracting HMW DNA from standard Dounce homogenizer tissue using Nanobind kits

Description

This procedure can be used to extract HMW DNA from animal tissues.

Procedure & checklist — Extracting HMW DNA from animal tissue using TissueRuptor

Description

This procedure can be used to extract HMW DNA from animal tissues using TissueRuptor.

Procedure & checklist — Extracting HMW DNA from Aplysia tissue using Nanobind kits

Description

This procedure can be used to isolate HMW DNA from the mollusk Aplysia.  

Procedure & checklist — Extracting HMW DNA from black mystery snail tissue using Nanobind kits

Description

This procedure can be used to isolate HMW DNA from frozen foot tissue of black mystery snail which is a freshwater snail.