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Documentation + protocols

Welcome to the PacBio Documentation page. The table below allows you to search for and download the latest guides, protocols, product information, and more. For any questions about our documentation, please contact us.

April 22, 2022

Procedure & checklist – Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

  • Procedure & Checklist
  • Sequel II System, Sequel IIe System
  • Template Preparation

This procedure can be used to construct amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio Sequel II and IIe systems. Amplicons may be barcoded during PCR, during library prep with SMRTbell barcoded adapters, or by both methods to create nested barcode combinations.
November 15, 2021

Procedure & Checklist – Preparing Multiplexed Microbial Libraries Using SMRTbell Express Template Prep Kit 2.0

  • Procedure & Checklist
  • Sequel II System, Sequel IIe System, Sequel System
  • Template Preparation

This streamlined procedure describes how to prepare multiplexed SMRTbell libraries of microbial genomes for sequencing on the Sequel System using the SMRTbell Express Template Preparation Kit 2.0. Up to sixteen genomes can be multiplexed on the Sequel System and up to 48 genomes can be multiplexed on the Sequel II System.
November 15, 2021

Procedure & Checklist – Preparing SMRTbell Libraries using PacBio Barcoded Overhang Adapters for Multiplexing Amplicons

  • Procedure & Checklist
  • Sequel II System, Sequel IIe System, Sequel System
  • Template Preparation

The procedure describes a workflow for quickly constructing SMRTbell libraries from PCR products using PacBio Barcoded Overhang Adapters and SMRTbell Express Template Prep Kit 2.0. In this workflow, a barcode is introduced to each amplicon through ligation with a hairpin adapter containing a 16-bp barcode. Once barcoded, amplicons can be pooled and purified for sequencing.
November 15, 2021

Procedure & Checklist – Preparing HiFi Libraries from Low DNA Input Using SMRTbell Express Template Prep Kit 2.0

  • Procedure & Checklist
  • Sequel II System, Sequel IIe System, Sequel System
  • Template Preparation

This streamlined procedure can be used to prepare SMRTbell libraries using Express Template Prep Kit 2.0 and gDNA as low as 150 ng. This procedure is applicable for the Sequel and Sequel II System and enables multiplexing a maximum of 2 small genomes (up to 600 Mb/genome) on the Sequel II System.
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