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Procedure & checklist — Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio systems.

Procedure & checklist — Generating PureTarget repeat expansion panel libraries

Description

This procedure describes the workflow for generating PureTarget repeat expansion libraries.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the Hamilton NIMBUS Presto system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Apex system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human blood using the Thermo Fisher KingFisher Apex robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Duo Prime system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Thermo Fisher KingFisher Duo Prime robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Flex system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Thermo Fisher KingFisher Flex robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the Hamilton NIMBUS Presto system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 200 μL of human whole blood using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the KingFisher Apex system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb DNA from 200 μL of human whole blood using the Thermo Fisher KingFisher Apex robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the KingFisher Duo Prime system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 200 μL of human whole blood using the Thermo Fisher KingFisher Duo Prime robotic instrument. This protocol requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 µL human whole blood on the KingFisher Flex system

Description

This protocol describes the extraction of HMW (50–300 kb) DNA from 200 µL of human whole blood using the Thermo Fisher KingFisher Flex. It is recommended for HiFi sequencing. This protocol requires the Nanobind HT CBB kit (102- 762-700).

Procedure & checklist — Removing short DNA fragments with the Short Read Eliminator (SRE) kit

Description

This procedure describes the workflow for DNA size selection to remove molecules <10 kb using the SRE kit on high molecular weight (HMW) DNA before shearing and library preparation.

Procedure & checklist — Preparing multiplexed whole genome and amplicon libraries using the HiFi plex prep kit 96

Description

This procedure describes the workflow for constructing multiplexed whole genome sequencing (WGS) and amplicon libraries using the HiFi plex prep kit 96 for sequencing on PacBio systems. The HiFi plex prep kit 96 supports the multiplexing of 24 samples or more.

Procedure & checklist — Preparing whole genome libraries using the HiFi prep kit 96

Description

This procedure describes the high throughput workflow for constructing whole genome sequencing (WGS) libraries from genomic DNA using the SRE HT kit, HiFi prep kit 96 and Revio polymerase kit 96.

Procedure & checklist — Extracting HMW DNA from cultured adherent cells using Nanobind kits

Description

This procedure can be used to extract HMW DNA from cultured adherent cells.

Procedure & checklist — Extracting HMW DNA from Gram-negative bacteria using Nanobind kits

Description

This procedure can be used to extract HMW DNA from Gram-negative bacteria.