September 22, 2019  |  

Research benefits of storing genitourinary samples: 16S rRNA sequencing to evaluate vaginal bacterial communities.

Authors: Wagner, Josef and Kerry-Barnard, Sarah and Sadiq, S T and Oakeshott, Pippa

Using well-characterised, but old and carefully frozen genital tract research samples may be a cost-effective way of performing metagenomic studies, but risks loss of low abundance (but relevant) bacterial species DNA. Moi et al.1 used 16S rRNA and UreDNA sequencing to detect ureaplasmas in frozen urine samples collected from 362 men with NGU in 2010–2011. They found that urethral inflammatory responses to ureaplasmas were less severe than to Chlamydia trachomatis and Mycoplasma genitalium.

Journal: International journal of STD & AIDS
DOI: 10.1177/0956462416689441
Year: 2017

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