July 7, 2019  |  

LongISLND: in silico sequencing of lengthy and noisy datatypes.

Authors: Lau, Bayo and Mohiyuddin, Marghoob and Mu, John C and Fang, Li Tai and Bani Asadi, Narges and Dallett, Carolina and Lam, Hugo Y K

LongISLND is a software package designed to simulate sequencing data according to the characteristics of third generation, single-molecule sequencing technologies. The general software architecture is easily extendable, as demonstrated by the emulation of Pacific Biosciences (PacBio) multi-pass sequencing with P5 and P6 chemistries, producing data in FASTQ, H5, and the latest PacBio BAM format. We demonstrate its utility by downstream processing with consensus building and variant calling.LongISLND is implemented in Java and available at http://bioinform.github.io/longislnd CONTACT: [email protected] information: Supplementary data are available at Bioinformatics online.© The Author 2016. Published by Oxford University Press.

Journal: Bioinformatics
DOI: 10.1093/bioinformatics/btw602
Year: 2016

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