Alternative splicing can increase the complexity of the transcriptome and proteome. The most common mechanism of alternative splicing in plants is intron retention (IR), and the expression levels of IR isoforms can be differentially regulated when facing abiotic stress. The full-length transcriptome of the medicinal plant Andrographis paniculata was sequenced using both Illumina- and SMRT-based RNA-seq and a total of 4846 IR isoforms were identified. The expression levels of 310/296 IR isoforms were up-regulated, and 629/659 IR isoforms were down-regulated at 24?h/48?h after methyl jasmonate (MeJA) treatment, respectively. In the (E,E,E)-geranylgeranyl diphosphate (GGPP) biosynthesis pathway which contributes to the andrographolide biosynthesis, eight genes were alternatively spliced, resulting in a total of 25 isoforms, of which 12 are IR isoforms. After MeJA treatment, four of these IR isoforms showed significant differential expression. RT-PCR and qRT-PCR experiments confirmed the existence of five IR isoforms. This research deepens our understanding of the A. paniculata transcriptome and can assist in the future study of andrographolide biosynthesis.
Journal: Industrial Crops and Products