September 22, 2019  |  

First draft genome assembly of the Argane tree (Argania spinosa)

Authors: Khayi, S and Azza, NE and Gaboun, F and Pirro, S and Badad, O and Claros, MG and Lightfoot, DA and Unver, T and Chaouni, B and Merrouch, R and Rahim, B and Essayeh, S and Ganoudi, M and Abdelwahd, R and Diria, G and Mdarhi, MA and Labhilili, M and Iraqi, D and Mouhaddab, J and Sedrati, H and Memari, M and Hamamouch, N and Alché, JdD and Boukhatem, N and Mrabet, R and Dahan, R and Legssyer, A and Khalfaoui, M and Badraoui, M and Van de Peer, Y and Tatusova, T and El Mousadik, A and Mentag, R and Ghazal, H

Background: The Argane tree (Argania spinosa L. Skeels) is an endemic tree of southwestern Morocco that plays an important socioeconomic and ecologic role for a dense human population in an arid zone. Several studies confirmed the importance of this species as a food and feed source and as a resource for both pharmaceutical and cosmetic compounds. Unfortunately, the argane tree ecosystem is facing significant threats from environmental changes (global warming, over-population) and over-exploitation. Limited research has been conducted, however, on argane tree genetics and genomics, which hinders its conservation and genetic improvement. Methods: Here, we present a draft genome assembly of A. spinosa. A reliable reference genome of A. spinosa was created using a hybrid de novo assembly approach combining short and long sequencing reads. Results: In total, 144 Gb Illumina HiSeq reads and 7.2 Gb PacBio reads were produced and assembled. The final draft genome comprises 75 327 scaffolds totaling 671 Mb with an N50 of 49 916 kb. The draft assembly is close to the genome size estimated by k-mers distribution and covers 89% of complete and 4.3 % of partial Arabidopsis orthologous groups in BUSCO. Conclusion: The A. spinosa genome will be useful for assessing biodiversity leading to efficient conservation of this endangered endemic tree. Furthermore, the genome may enable genome-assisted cultivar breeding, and provide a better understanding of important metabolic pathways and their underlying genes for both cosmetic and pharmacological purposes.

Journal: F1000Research
DOI: 10.12688/f1000research.15719.1
Year: 2018

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