ARTISAN PCR: rapid identification of full-length immunoglobulin rearrangements without primer binding bias.

Authors: Koning, Marvyn T and Kielbasa, Szymon M and Boersma, Vesna and Buermans, Henk P J and van der Zeeuw, Sander A J and van Bergen, Cornelis A M and Cleven, Arjen H G and Kluin, Philip M and Griffioen, Marieke and Navarrete, Marcelo A and Veelken, Hendrik

B cells recognize specific antigens by their membrane-bound B-cell receptor (BCR). Functional BCR genes are assembled in pre-B cells by recombination of the variable (V), diversity (D) and joining (J) genes [V(D)J recombination]. When B cells participate in germinal centre reactions, non-templated point mutations are introduced into BCR genes by somatic hypermutation (SHM) (Rajewsky, 1996). V(D)J recombination and SHM create virtually unlimited BCR repertoires.

Journal: British journal of haematology
DOI: 10.1111/bjh.14180
Year: 2016

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