April 21, 2020  |  

A reference-grade wild soybean genome.

Authors: Xie, Min and Chung, Claire Yik-Lok and Li, Man-Wah and Wong, Fuk-Ling and Wang, Xin and Liu, Ailin and Wang, Zhili and Leung, Alden King-Yung and Wong, Tin-Hang and Tong, Suk-Wah and Xiao, Zhixia and Fan, Kejing and Ng, Ming-Sin and Qi, Xinpeng and Yang, Linfeng and Deng, Tianquan and He, Lijuan and Chen, Lu and Fu, Aisi and Ding, Qiong and He, Junxian and Chung, Gyuhwa and Isobe, Sachiko and Tanabata, Takanari and Valliyodan, Babu and Nguyen, Henry T and Cannon, Steven B and Foyer, Christine H and Chan, Ting-Fung and Lam, Hon-Ming

Efficient crop improvement depends on the application of accurate genetic information contained in diverse germplasm resources. Here we report a reference-grade genome of wild soybean accession W05, with a final assembled genome size of 1013.2?Mb and a contig N50 of 3.3?Mb. The analytical power of the W05 genome is demonstrated by several examples. First, we identify an inversion at the locus determining seed coat color during domestication. Second, a translocation event between chromosomes 11 and 13 of some genotypes is shown to interfere with the assignment of QTLs. Third, we find a region containing copy number variations of the Kunitz trypsin inhibitor (KTI) genes. Such findings illustrate the power of this assembly in the analysis of large structural variations in soybean germplasm collections. The wild soybean genome assembly has wide applications in comparative genomic and evolutionary studies, as well as in crop breeding and improvement programs.

Journal: Nature communications
DOI: 10.1038/s41467-019-09142-9
Year: 2019

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