June 1, 2021

Targeted sequencing of genes from soybean using NimbleGen SeqCap EZ and PacBio SMRT Sequencing

Author(s): Gu, Jenny and Eng, Kevin and Sethuraman, Anand and Raterman, Denise and Richmond, Todd and Kujawa, Steve

Full-length gene capture solutions offer opportunities to screen and characterize structural variations and genetic diversity to understand key traits in plants and animals. Through a combined Roche NimbleGen probe capture and SMRT Sequencing strategy, we demonstrate the capability to resolve complex gene structures often observed in plant defense and developmental genes spanning multiple kilobases. The custom panel includes members of the WRKY plant-defense-signaling family, members of the NB-LRR disease-resistance family, and developmental genes important for flowering. The presence of repetitive structures and low-complexity regions makes short-read sequencing of these genes difficult, yet this approach allows researchers to obtain complete sequences for unambiguous resolution of gene models. This strategy has been applied to genomic DNA samples from soybean coupled with barcoding for multiplexing.

Organization: PacBio
Year: 2016

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