Pacific Biosciences’ Software Upgrade Enhances De Novo Genome Assembly, Variant Calling and cDNA Transcript Analysis
Tuesday, January 29, 2013
SMRT Analysis 1.4 includes a new hierarchical de novo genome assembly process (HGAP), which allows researchers to assemble entire microbial and fungal genomes solely using PacBio long reads. As a result, users can generate better assemblies with a single library preparation and fewer SMRT® Cells than previous approaches that also required short-read sequencing technologies or circular consensus sequencing.
SMRT Analysis 1.4 also incorporates a new multi-read consensus algorithm called Quiver to determine the finished genome sequence with exceptional accuracy. Quiver can provide greater than 99.999% consensus accuracy for both resequencing and de novo assembly applications.
“The extra-long sequence reads and the subsequent highly accurate de novo assemblies produced by the PacBio RS through the latest software enhancements have immensely improved our ability to discover and understand novel genomes cost-effectively,” said
The software upgrade also includes support for analyzing full-length cDNA transcripts, facilitating understanding of transcription, gene structure and alternative splicing. Unlike any other sequencing technology, SMRT Sequencing can span entire cDNA transcripts with a single read, revealing the complete exonic structure of transcripts.
Additional features of the new SMRT Analysis 1.4 upgrade include:
Support for analysis of barcoded samples;
Improvements to the tools for analyzing and visualizing bacterial methylomes, a capability unique to SMRT Sequencing;
Enhanced user and group permissions to help sequencing centers and core labs control what data is presented to end users.
“We are committed to continually improving the performance of the PacBio RS, in part through developing software enhancements designed to leverage the unique characteristics of our system,” said
The new SMRT Analysis software upgrade is available for download from Pacific Biosciences’ community website, along with sample data and protocols for cDNA sample preparation and barcoding. To access the software, data, and documentation, visit www.smrtcommunity.com.
For more information on the new SMRT Analysis software and the PacBio RS High Resolution Genetic Analyzer, please visit www.pacificbiosciences.com.
About Pacific Biosciences
Maurissa MessierFor Pacific Biosciences 760.539.7417 email@example.com Investors: Trevin RardPacific Biosciences 650.521.8450 firstname.lastname@example.org
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