Single Molecule Real Time (SMRT®) sequencing products, today
announced the first PacBio® RS compatible products
available through its Partner Program. These solutions complement and
enhance key aspects of the PacBio RS sequencing workflow,
providing customers with integrated workflows from sample preparation to
The PacBio RS is a revolutionary DNA sequencing system that
reveals new biological insights by incorporating novel, single molecule
sequencing techniques, advanced analytics, and long read lengths. The
following products are now fully compatible with the PacBio RS
data and workflow to provide whole product solutions for de novo
whole genome assembly and targeted sequencing.
For shearing of genomic DNA:
g-TUBE™ from Covaris, a single-use consumable that enables scientists
to shear genomic DNA into selected fragment sizes ranging from 6kb to
20kb. Up to 24 samples can be processed in several minutes and the
fragments can go directly into the SMRTbell™ template preparation
For target enrichment:
Access Array™ System from Fluidigm, a target enrichment platform
designed for resequencing selected regions of a genome. When combined
with PacBio barcodes, the system can generate 48 amplicons for each of
48 samples and go directly into the SMRTbell template preparation
SureSelect™ Target Enrichment System from Agilent Technologies
improves the cost and process efficiency of the sequencing workflow.
The system has demonstrated the ability to capture targeted fragments
up to 2kb in length that are ready for SMRTbell template preparation.
BG7 Bacterial Genome Annotation Service from Era7 Bioinformatics for
gene and RNA prediction from PacBio de novo assemblies.
Exemplar LIMS from Sapio Sciences has pre-loaded PacBio protocols and
offers a workflow-driven architecture and plugin framework for
automating sample preparation through sequencing and analysis.
The GenoLogics LIMS provides preconfigured workflows for many genomics
platforms including the PacBio RS. It provides the end-to-end
sample traceability that genomics labs require to ensure the quality
and integrity of their results.
CLC Genomics Workbench and CLC Genomics Server from CLC bio can
take alignments from the PacBio RS to call SNPs and perform
tertiary analysis, giving scientists an easy way to do functional
classification and filtering of SNPs and other genomic variations
using multiple data sources.
Partek® Flow™, with an intuitive user interface designed
with the biologist and informaticist in mind, provides increased
quality control, powerful statistics for variant detection and copy
number analysis, as well as dynamic visualizations for informative
views of data and biomarker identification of aligned PacBio reads.
SeqMan® NGen® from
DNASTAR, Inc., sequence
assembly software that can perform reference-based alignment of PacBio
data and call SNPs quickly and accurately on a desktop computer.
SeqMan NGen also easily integrates PacBio data with data from other
sequencing platforms for integrated sequence assembly projects, if
SNP & Variation Suite 7 (SVS) from Golden Helix, an integrated
collection of user-friendly, yet powerful tools for variant
classification, bioinformatic filtering, functional annotation,
visualization, and statistical analysis for quickly uncovering
statistically significant genotype/phenotype associations or causal
variants from PacBio variant calls.
For more information about these products, please visit the Pacific
Biosciences Partner Products website at www.pacb.com/partner_products.
have experience using a product developed out of the Partner Program.
“We have been using the g-TUBES from Covaris to prepare libraries for
use on the PacBio RS,” said
that they speed up the library preparation step.”
commented: “We are delighted with the progress we’ve made working with
these companies since we launched our Partner Program. The products that
are resulting from these collaborations further support our customers to
perform key applications on the PacBio RS such as targeted
sequencing and de novo whole genome assembly, and advance our
goal of providing whole product solutions.”
Pacific Biosciences partners, customers and scientists will be
presenting data generated with the PacBio RS and some of these
co-developed solutions at this week’s Advances in Genome Biology &
Technology (AGBT) conference in
please visit: www.pacb.com/agbt2012.
About Pacific Biosciences
Pacific Biosciences’ mission is to transform the way humankind acquires,
processes and interprets data from living systems through the design,
development and commercialization of innovative tools for biological
research. The company has developed a novel approach to studying the
synthesis and regulation of DNA, RNA and proteins. Combining recent
advances in nanofabrication, biochemistry, molecular biology, surface
chemistry and optics, Pacific Biosciences has created a powerful
technology platform called single molecule, real-time, or SMRT®,
technology. SMRT technology enables real-time analysis of biomolecules
with single molecule resolution, which has the potential to transform
the understanding of biological systems by providing a window into these
systems that has not previously been open for scientific study.
For more information about Pacific Biosciences, please visit www.pacb.com.
You can also follow the company on Twitter: www.twitter.com/pacbio.
This press release contains forward-looking statements. Forward-looking
statements may contain words such as “believe,” “may,” “estimate,”
“anticipate,” “continue,” “intend,” “expect,” “plan,” the negative of
these terms, or other similar expressions, and include the assumptions
that underlie such statements. Such statements include, but are not
limited to, statements regarding the Company’s SMRT technology. These
statements are subject to known and unknown risks and uncertainties that
could cause actual results to differ materially from those expressed or
implied by such statements, including but not limited to risks discussed
from time to time in documents
has filed with the
risks identified under the section captioned “Risk Factors” in its most
recently filed Quarterly Report on Form 10-Q. All forward-looking
statements are based on estimates, projections and assumptions as of the
date hereof. Pacific Biosciences undertakes no obligation to update any
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