Cold Spring Harbor Laboratory Scientists Produce High-Quality Maize Transcriptome with PacBio SMRT Sequencing
Friday, June 24, 2016
Led by CSHL scientists
Iso-Seq analysis of SMRT Sequencing data more than doubled the number of isoforms, corrected numerous previously mis-annotated gene models, and identified many novel genes and long non-coding RNAs. Additionally, the team showed that long reads are even more important than expected for transcriptome studies. The average transcript length in this project — almost 3 kb — is much longer than that from the previous maize annotation, highlighting a strong bias limitation in previous approaches.
“Although data from short-read sequencing have accumulated over recent years, they do not provide full-length sequence for each RNA, limiting their utility for defining alternatively spliced forms,” Wang et al. write. “In some cases, short-read sequencing generates low-quality transcripts, leading to incorrect annotations.”
“We congratulate the scientists at
More information is available in this blog post, and in the paper, “Unveiling the complexity of the maize transcriptome by single-molecule long-read sequencing.”
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