PacBio Literature

Kinnex offers full-length isoform discovery and abundance information, cell type-specific isoform discovery, and species-level metagenomics community identification that are difficult to capture with short reads.

The Revio™ system empowers HiFi sequencing. It adds affordability, high throughput, and ease of use to a foundation of long reads, exceptional accuracy, and direct methylation detection.

Highlighting publications using Iso-Seq in cancer research to demonstrate how bulk and single-cell Iso- Seq data detects fusion genes, identifies novel isoforms that might serve as neoepitopes for vaccine candidates, and traces clonal evolution.

Reveal the complexity of the human genome to power human disease research — With the advancement of highly accurate long-read sequencing, missing pieces could be resolved and fully complete the genome. PacBio HiFi sequencing gave rise to this achievement and has dramatically improved understanding of the human genome by resolving gaps that are unattainable by other sequencing methods, accurately mapping complex regions, and enabling more contiguous genome assemblies.

This technical note describes the procedure for targeted enrichment using IDT xGen Hyb and Wash kit v3 for KinnexTM full-length RNA libraries.

PacBio® HiFi sequencing enables greater variant detection in more regions of the genome than alternative technologies, providing exceptional insight at any depth. Choosing the right level of sequencing coverage is a critical first step for project planning

This technical note demonstrates the HiFi sequencing performance of saliva samples collected and stabilized with DNA Genotek Oragene devices and extracted using PacBio Nanobind kits.

This application note details a wide array of bioinformatics tools for full length- isoform sequencing across isoform classification and annotation, fusion transcript discovery, variant calling, transcript assembly, differential transcript analysis, differential splicing usage, and transcript visualization.

This technical note describes the experimental conditions of a modified workflow for preparing PacBio® Kinnex single-cell libraries with single-cell or single-nucleus cDNA generated using Parse Biosciences Evercode Whole Transcriptome products, the WT and WT Mini kits. This workflow has not been tested using the Evercode WT Mega kit.

This technical note provides guidance for size selection of whole genome sequencing (WGS) HiFi libraries for sequencing on Sequel II or Revio systems.

PacBio® Nanobind® HT kits enable automation of DNA extraction, the first step of an end-to-end workflow that harnesses the power of HiFi sequencing. Nanobind HT kits are designed for whole human blood and mammalian cell samples and are compatible with four robotic platforms.

The all-new PacBio® Nanobind® PanDNA kit consolidates the capabilities of our existing sample-specific offerings into a single solution for DNA extraction. This kit provides users with an all-in-one extraction experience that can be used with a wide range of sample types. The PanDNA kit supports high-quality extraction from cultured cells, bacteria, whole blood, tissue, plant nuclei, and insect samples.

Overview of HiFi application options

This technical note demonstrates HiFi sequencing performance from HMW insect DNA extracted using the Nanobind PanDNA kit.

PacBio HiFi prep kits offer scalable library prep solutions for projects and genomes of all sizes.