PacBio Literature

PacBio® HiFi sequencing enables greater variant detection in more regions of the genome than alternative technologies, providing exceptional insight at any depth. Choosing the right level of sequencing coverage is a critical first step for project planning

This technical note demonstrates the HiFi sequencing performance of saliva samples collected and stabilized with DNA Genotek Oragene devices and extracted using PacBio Nanobind kits.

This application note details a wide array of bioinformatics tools for full length- isoform sequencing across isoform classification and annotation, fusion transcript discovery, variant calling, transcript assembly, differential transcript analysis, differential splicing usage, and transcript visualization.

This technical note describes the experimental conditions of a modified workflow for preparing PacBio® Kinnex single-cell libraries with single-cell or single-nucleus cDNA generated using Parse Biosciences Evercode Whole Transcriptome products, the WT and WT Mini kits. This workflow has not been tested using the Evercode WT Mega kit.

This technical note provides guidance for size selection of whole genome sequencing (WGS) HiFi libraries for sequencing on Sequel II or Revio systems.

Detect, track, and characterize pathogens to a new standard of completeness and precision. PacBio® solutions offer unmatched accuracy and long read lengths to support a wide range of pathogen surveillance applications.

PacBio® Nanobind® HT kits enable automation of DNA extraction, the first step of an end-to-end workflow that harnesses the power of HiFi sequencing. Nanobind HT kits are designed for whole human blood and mammalian cell samples and are compatible with four robotic platforms.

The all-new PacBio® Nanobind® PanDNA kit consolidates the capabilities of our existing sample-specific offerings into a single solution for DNA extraction. This kit provides users with an all-in-one extraction experience that can be used with a wide range of sample types. The PanDNA kit supports high-quality extraction from cultured cells, bacteria, whole blood, tissue, plant nuclei, and insect samples.

Overview of HiFi application options

This technical note demonstrates HiFi sequencing performance from HMW insect DNA extracted using the Nanobind PanDNA kit.

PacBio HiFi prep kits offer scalable library prep solutions for projects and genomes of all sizes.

The Kinnex 16S rRNA kit increases throughput by 12-fold using the MAS- Seq concatenation technology. We show that more read depth allows for higher multiplexing capacity (up to 1,536-plex) and allows more identified species per sample. Coupled with flexible multiplexing strategies and a SMRT® Link bioinformatics workflow, users can now achieve cost-effective full-length 16S rRNA gene sequencing for species- and strain-level identification for microbiome studies.

The outstanding accuracy of HiFi long-read sequencing allows you to get the most out of each Revio run. Discover how you can access more information at reduced cost and higher throughput for each of your sequencing projects.

In this whitepaper, we discuss the features of bulk and single-cell isoform sequencing with the Iso-Seq method and their applications in human genomics.

The LightBench from Yourgene Health uses Ranger Technology to deliver accurate size selection for whole genome sequencing and includes the ability to perform fragment length analysis for additional quality control. With an automated workflow to optimize walk-away time, the LightBench offers a scalable size selection solution for the preparation of SMRTbell gDNA libraries for HiFi sequencing.