PacBio Literature

Comprehensive genome sequencing data integrated with chromatin interaction information is crucial for complete, chromosome-scale genome assembly. CiFi (pronounced “Sci-Fi”) is a new application developed by the sequencing community that integrates chromatin conformation capture (3C) with PacBio HiFi long-read sequencing to generate highly accurate, multi-contact reads.

Unravel the complexity of plant and animal genomes with HiFi sequencing to harness agrigenomics insights for breeding, crop protection, and genome engineering.

This application note demonstrates that DNA extracted from whole blood and saliva samples using the Nanobind kits yields high-quality HiFi sequencing data.

Unravel the complexity of plant and animal biology for any species with HiFi sequencing. Discover more about species and populations with more accurate genomes, transcriptomes, and epigenomes.

PacBio® provides cutting-edge sequencing that allows scientists to unlock the genetic diversity of all species on earth. These tools can fuel discoveries in agriculture, biodiversity, and environmental health, and build toward a sustainable future.

In this whitepaper, we discuss the features of full- length and single-cell isoform sequencing with the Iso-Seq method and their applications in plant and animal research. We show how combining the Iso-Seq method with Kinnex kits further increases throughput, making PacBio long reads a cost-effective RNA sequencing solution.

The Kinnex single-cell RNA kit is compatible with cDNA generated using the Visium HD 3' Spatial Gene Expression assay for creating a sequencing-ready library that can run on PacBio sequencing systems.

SMRT Link Cloud is a free, PacBio-hosted version of SMRT Link that removes the need for IT maintenance.

With HiFi target enrichment you can easily and cost-effectively sequence just the genes you care about at scale. Design custom gene panels with our partners at Twist, sequence them with long and accurate HiFi reads, and get all the benefits you expect from HiFi reads: comprehensive detection of single nucleotide variants, structural variants, and indels. HiFi target enrichment is the only technology that can resolve complex gene families like HLA or Cytochrome P450 genes with haplotype resolution.

Other sequencing methods suffer from shortcomings like an inability to capture the backbone sequence or low accuracy in difficult genomic regions such as homopolymers or regions of high GC content. PacBio® HiFi sequencing overcomes these limitations and allows you to capture the full plasmid sequence, including backbone and difficult regions.

The whole genome sequencing (WGS) application requires an up-front mechanical DNA shearing step to transform genomic DNA into fragment sizes appropriate for HiFi sequencing. With the Hamilton Microlab Prep system, this step can be performed in as little as 22 minutes for 24 samples at the cost of a pipette tip per sample. Here we describe the required equipment and consumables, best practices, and procedure for shearing DNA on the Microlab Prep system.

Fiber-seq is a powerful new long-read whole genome sequencing assay that overcomes the challenges imposed by short-read chromatin sequencing.

With PacBio long reads you can easily and cost-effectively sequence the full length of amplicons that target genes or regions of interest, from several hundred base pairs to kilobase scale. Highly accurate HiFi reads allow you to target across types of variation, from single nucleotide variants and indels, to structural variants. It also enables unambiguous haplotype resolution through direct phasing. HiFi sequencing can reveal biology that other technologies miss, like low-complexity regions, repeat expansions, GC-rich promoter regions, and insertion sites of transposable elements.

HiFi systems brochure

Application brief — What can you do with one SMRT Cell