Building Better Genomes.
Enabling Breakthrough Discovery.
PacBio HiFi reads provide both long read lengths (up to 25 kb) and high accuracy (>99.9%) to quickly and affordably generate contiguous, complete, and correct de novo genome assemblies of even the most complex genomes.
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Use this workflow to generate high-quality de novo assemblies quickly and affordably with HiFi reads. You can assemble up to a 2 Gb genome in a single SMRT Cell 8M or scale up for larger genomes and run up to 200 samples (2 Gb) per year, per Sequel II or IIe System. |
See the Complete Workflow
Studying Small-bodied Organisms?
Generate high-quality genome assemblies for single, wild-caught individuals from as little as 5 ng of input DNA
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To get a quote and get started on your genome project, contact one of our Certified Service Providers or a PacBio scientist. For information on acquiring a Sequel IIe System, please contact our sales team.
Selected Resources
- Kingan, Sarah B et al. (2019) A high-quality genome assembly from a single, field-collected spotted lanternfly (Lycorma delicatula) using the PacBio Sequel II system GigaScience
- Tørresen, Ole K et al. (2019) Tandem repeats lead to sequence assembly errors and impose multi-level challenges for genome and protein databases. Nucleic acids research
- He, Yaoxi et al. (2019) Long-read assembly of the Chinese rhesus macaque genome and identification of ape-specific structural variants. Nature communications
- Zhou, Yongfeng et al. (2019) The population genetics of structural variants in grapevine domestication. Nature plants
- Meier, Matthew J et al. (2019) Whole Genome Sequencing of the Mutamouse Model Reveals Strain- and Colony-Level Variation, and Genomic Features of the Transgene Integration Site. Scientific reports
- Wenger, Aaron M et al. (2019) Accurate circular consensus long-read sequencing improves variant detection and assembly of a human genome. Nature biotechnology
- Liu, Qin et al. (2019) A chromosomal-level genome assembly for the insect vector for Chagas disease, Triatoma rubrofasciata. GigaScience
- Van de Weyer, Anna-Lena et al. (2019) A Species-Wide Inventory of NLR Genes and Alleles in Arabidopsis thaliana. Cell
- Bickhart, Derek M et al. (2019) Assignment of virus and antimicrobial resistance genes to microbial hosts in a complex microbial community by combined long-read assembly and proximity ligation. Genome biology
- Jaworski, Coline C et al. (2019) Chromosome-level hybrid de novo genome assemblies as an attainable option for non-model organisms BioRxiv
- Low, Wai Yee et al. (2019) Haplotype-Resolved Cattle Genomes Provide Insights Into Structural Variation and Adaptation BioRxiv
- Yang, Zhaoen et al. (2019) Extensive intraspecific gene order and gene structural variations in upland cotton cultivars. Nature communications
- McCartney, Michael A. et al. (2019) The Genome of the Zebra Mussel, Dreissena polymorpha: A Resource for Invasive Species Research BioRxiv
- Zan, Jindong et al. (2019) A microbial factory for defensive kahalalides in a tripartite marine symbiosis. Science
- Warr, Amanda et al. (2019) An improved pig reference genome sequence to enable pig genetics and genomics research BioRxiv
- Chen, Zelin et al. (2019) De novo assembly of the goldfish (Carassius auratus) genome and the evolution of genes after whole-genome duplication. Science advances
- Yang, Ning et al. (2019) Genome assembly of a tropical maize inbred line provides insights into structural variation and crop improvement. Nature genetics
- Sánchez-Pérez, R et al. (2019) Mutation of a bHLH transcription factor allowed almond domestication. Science
- Yoshimura, Jun et al. (2019) Recompleting the Caenorhabditis elegans genome. Genome research
- Chang, Ching-Ho et al. (2019) Islands of retroelements are major components of Drosophila centromeres PLoS biology
- Zhuang, Weijian et al. (2019) The genome of cultivated peanut provides insight into legume karyotypes, polyploid evolution and crop domestication. Nature genetics
- Hotaling, Scott et al. (2019) The rising tide of high-quality genomic resources. Molecular ecology resources
- Kronenberg, Zev N. et al. (2019) Extended haplotype phasing of de novo genome assemblies with FALCON-Phase BioRxiv
- Ananthasayanam, Sudhakar et al. (2019) First near complete haplotype phased genome assembly of River buffalo (Bubalus bubalis) BioRxiv
- Chen, Wenbo et al. (2019) Genome sequence of the corn leaf aphid (Rhopalosiphum maidis Fitch). GigaScience
- Zhao, Dongyan et al. (2019) A chromosomal-scale genome assembly of Tectona grandis reveals the importance of tandem gene duplication and enables discovery of genes in natural product biosynthetic pathways. GigaScience
- Beaulaurier, John et al. (2019) Deciphering bacterial epigenomes using modern sequencing technologies. Nature reviews. Genetics
- Masonbrink, Rick et al. (2019) The genome of the soybean cyst nematode (Heterodera glycines) reveals complex patterns of duplications involved in the evolution of parasitism genes. BMC genomics
- Kingan, Sarah et al. (2019) A high-quality de novo genome assembly from a single mosquito using PacBio sequencing Genes
- Laverty, Kaitlin U et al. (2019) A physical and genetic map of Cannabis sativa identifies extensive rearrangements at the THC/CBD acid synthase loci. Genome research
- Low, Wai Yee et al. (2019) Chromosome-level assembly of the water buffalo genome surpasses human and goat genomes in sequence contiguity. Nature communications
- Ruan, Jue et al. (2019) Fast and accurate long-read assembly with wtdbg2 BioRxiv
- Hoff, Siv Nam Khang et al. (2019) Long-read sequence capture of the haemoglobin gene clusters across codfish species. Molecular ecology resources
- Formenti, Giulio et al. (2019) SMRT long reads and Direct Label and Stain optical maps allow the generation of a high-quality genome assembly for the European barn swallow (Hirundo rustica rustica). GigaScience
- Bell, Zachary W et al. (2019) Urotensin-related gene transcripts mark developmental emergence of the male forebrain vocal control system in songbirds. Scientific reports
- Poster: Baybayan, Primo et al. (2020) New advances in SMRT Sequencing facilitate multiplexing for de novo and structural variant studies
- Poster: Galvin, Brendan et al. (2020) A high-quality PacBio insect genome from 5 ng of input DNA
- Poster: Kingan, Sarah B. et al. (2020) Beyond Contiguity: Evaluating the accuracy of de novo genome assemblies
- Poster: Kingan, Sarah B. et al. (2020) Every species can be a model: Reference-quality PacBio genomes from single insects
- Poster: Vierra, M. et al. (2019) Structural variant detection in crops using low-fold coverage long-read sequencing
- Poster: Duncan, Turner et al. (2019) A low DNA input protocol for high-quality PacBio de novo genome assemblies
- Poster: Ekholm, Jenny et al. (2019) Streamlines SMRTbell library generation using addition-only, single tube strategy for all library types reduces time to results
- Poster: Baybayan, Primo et al. (2019) A high-quality de novo genome assembly from a single mosquito using PacBio sequencing
- Poster: Kingan, Sarah B. et al. (2019) A low DNA input protocol for high-quality PacBio de novo genome assemblies from single invertebrate individuals
- Poster: Peluso, Paul et al. (2019) Single molecule high-fidelity (HiFi) Sequencing with >10 kb libraries
- Poster: Blethrow, J. Sisneros, N. et al. (2018) Best practices for whole genome sequencing using the Sequel System
- Poster: Korlach, Jonas et al. (2018) High-quality de novo genome assembly and intra-individual mitochondrial instability in the critically endangered kakapo
- Poster: Heiner, Cheryl et al. (2018) Multiplexed complete microbial genomes on the Sequel System
- Poster: Vierra, Michelle et al. (2018) Characterizing the pan-genome of maize with PacBio SMRT Sequencing
- Poster: Concepion, C., et al. (2018) De novo assembly and preliminary annotation of the Schizocardium californicum genome
- Poster: Concepcion, G., et al. (2018) Structural variant detection in crops using PacBio SMRT Sequencing
- Poster: Kingan, Sarah et al. (2017) A high-quality genome assembly of SMRT sequences reveals long range haplotype structure in the diploid mosquito Aedes aegypti
- Poster: Korlach, Jonas et al. (2017) De novo PacBio long-read assembled avian genomes correct and add to genes important in neuroscience and conservation research
- Poster: Kingan, S. et al. (2017) A high-quality genome assembly of SMRT Sequences reveals long-range haplotype structure in the diploid mosquito Aedes aegypti
- Poster: Warr, A. et al. (2017) High-quality, highly contiguous re-assembly of the pig genome
- Poster: Concepcion, G. et al. (2017) Phased diploid genome assembly with single-molecule real-time sequencing
- Poster: Roach, M. et al. (2016) Assessing diversity and clonal variation of Australia’s grapevine germplasm: Curating the FALCON-Unzip Chardonnay de novo genome assembly
- Poster: Baybayan, Primo et al. (2016) Application specific barcoding strategies for SMRT Sequencing
- Poster: Ranade, Swati et al. (2016) Immune regions are no longer incomprehensible with SMRT Sequencing
- Poster: Seetin, Matthew et al. (2016) Long-read assembly of the Aedes aegypti Aag2 cell line genome resolves ancient endogenous viral elements
- Poster: Heiner, Cheryl et al. (2016) Low-input long-read sequencing for complete microbial genomes and metagenomic community analysis
- Poster: Chin, Jason et al. (2016) Un-zipping diploid genomes – revealing all kinds of heterozygous variants from comprehensive hapltotig assemblies
- Presentation: Chin, Jason et al. (2016) Diploid genome assembly and comprehensive haplotype sequence reconstruction
- Poster: Seetin, Matthew G et al. (2016) Long-read assembly of the Aedes aegypti Aag2 cell line genome resolves ancient endogenous viral elements
- Poster: Muehling, Jill R et al. (2016) SMRT Sequencing of the alala genome
- Poster: Lee, Hayan et al. (2015) The resurgence of reference quality genome
- Poster: Rank, David et al. (2015) Best practices for whole-genome de novo sequencing with long-read SMRT Sequencing.
- Presentation: Schatz, Michael et al. (2015) The resurgence of reference quality genome sequence.
- Presentation: VanBuren, Robert et al. (2015) De novo assembly of a complex panicoid grass genome using ultra-long PacBio reads with P6C4 chemistry
- Presentation: Strickler, Susan R et al. (2015) Genome assembly strategies of the recent polyploid, Coffea arabica.
- Blethrow, Justin and Smith, Melissa L. and Vinnere Pettersson, Olga (2021) Webinar: Unleashing the Power of HiFi Sequencing – How the Sequel IIe System Removes Barriers and Empowers Life Scientists
- Miller, Dave and Hernandez, Alvaro (2021) Creating Core Demand with HiFi Sequencing
- Wenger, Aaron (2021) Not All Long Reads Are Equal – High Accuracy Sets PacBio Apart
- Miller, David and Smith, Melissa Laird (2021) The Long and Short of Sequencing – Why HiFi Reads are the Future
- Becker, Aaron and Ekholm, Jenny and Harris, Charlotte and Kingham, Brewster and Vinnere Pettersson, Olga (2021) SMRT Sequencing as a Service – How to Bring Long-Read Technology to Your Core Lab
- Wang, Bo (2021) PagBio Day: Comparative Genomic Analysis in Sorghum Highlights the Extent of Structural Variations and Sugarcane Aphid Resistance Genes
- Vierra, Michelle and Lewin, Haris (2021) PagBio Day: New Discoveries in Plant and Animal Sciences
- (2020) Introduction to highly accurate long-read sequencing (HiFi Sequencing)
- Balacco, Jennifer (2020) Virtual Global Summit: Adventures towards error-free genomes
- Lawniczak, Mara (2020) Virtual Global Summit: Anopheles and the journey from a reference genome to a thousand genomes to spatiotemporal genomic observatories
- Carbonell, Pablo (2020) Virtual Global Summit: Elucidating the origin of grapevine agronomic traits with PacBio genome assembly and Iso-Seq analysis
- Schmutz, Jeremy (2020) Virtual Global Summit: HiFi – Amazing Applications in Plant Genomes and Human Disease Research
- Chong, Tang (2020) Virtual Global Summit: HIT-scISOseq – High-throughput and high-accuracy single-cell full-length isoform sequencing
- Newell, Nicole (2020) Virtual Global Summit: Technical Tutorial – Choosing the best library prep for HiFi sequencing
- Hernandez, Alvaro (2020) Virtual Global Summit: Using the unique capabilities of the Sequel II System for de novo genome assembly and full-length 16S sequencing
- (2020) Video: HiFi Sequencing – Unlock your next great discovery
- Geib, Scott and Laumer, Christopher and Uliano da Silva, Marcela and Vierra, Michelle (2020) Webinar: No Organism Too Small – Build high-quality genome assemblies of small organisms with HiFi sequencing
- Uliano da Silva, Marcela (2020) SMRT Leiden: The impact of polishing on Darwin Tree of Life chromosome-level lepidopteran genome assemblies built with PacBio HiFi and Illumina Hi-C reads
- Jarvis, Erich (2020) SMRT Leiden: The Vertebrate genomes project: Sequencing life for a new biology
- Datema, Erwin (2020) SMRT Leiden: Ultra-fast comparative genomics of complex genomes at population scale
- Korlach, Jonas and Graves-Lindsay, Tina and Vierra, Michelle (2020) PacBio Workshop: Understanding the biology of genomes with HiFi sequencing
- Schmutz, Jeremy and Conesa, Ana and Gharbi, Karim and Kuo, Richard (2020) Customer Experience: HiFi reads help scientists explore genomes and transcriptomes
- Schmutz, Jeremy (2020) Customer Experience: PacBio HiFi reads improve genome sequencing at HudsonAlpha
- Korlach, Jonas (2020) Webinar: Long HiFi reads for high-quality genome assemblies
- Mars, Kristin and Laird Smith, Melissa and Vinnere Pettersson, Olga and Hardigan, Michael (2020) Webinar: Sequencing 101 – How long-read sequencing improves access to genetic information
- Oppert, Brenda (2020) AGBT Presentation: Feed the World – Developing genomic resources for insects as food
- Blaxter, Mark (2020) PAG Conference: Endless forms: Genomes from the Darwin Tree of Life Project
- Vierra, Michelle (2020) PAG Conference: PacBio update on products and HiFi applications
- Nijland, Bart (2020) PAG Conference: The impact of highly accurate PacBio sequence data on the assembly of a tetraploid rose
- Korlach, Jonas (2020) PAG Conference: Workshop introduction
- Bernberg, Erin (2019) User Group Meeting: Getting a read on one little worm with PacBio’s low DNA input workflow and the Agilent Femto Pulse System
- Korlach, Jonas (2019) User Group Meeting: Lowering input requirements
- Korlach, Jonas (2019) User Group Meeting: Sequencing chemistry & application updates
- Bernberg, Erin and Hotaling, Scott and Vierra, Michelle (2019) Webinar: Small Bodies, Big Genomes: Overcoming Large DNA Input Requirements for Long-Read Genome Assembly
- Kingan, Sarah and Carroll, Andrew (2019) Webinar: Variant calling and de novo genome assembly with PacBio HiFi reads
- Application Brief: Whole genome sequencing for de novo assembly – Best Practices (2021)
- Application Brochure: What can you do with one SMRT Cell? (2021)
- SMRT Sequencing Brochure: Delivering highly accurate long reads to drive discovery in life science (2020)
- Product Brochure: Sequel IIe System – Sequencing evolved (2020)
- Informational Guide: Understanding accuracy in DNA sequencing (2020)
- Application Note: Considerations for using the low and ultra-low DNA input workflows for whole genome sequencing (2020)
- Application Brochure: HiFi reads for highly accurate long-read sequencing (2020)
- Technical Note: Preparing DNA for PacBio HiFi sequencing – Extraction and quality control (2020)
- Informational Guide: Looking beyond the single reference genome to a pangenome for every species (2020)
- Infographic: A Genome Fit for a Giant – Sequencing the California redwood (2020)
- PacBio Certified Service Providers (2020)
- Case Study: Diving Deep – Revealing the mysteries of marine life with SMRT Sequencing (2019)
- Infographic: Gold-standard reference genomes accelerate science (2018)
- Technical Note: Preparing samples for PacBio whole genome sequencing for de novo assembly – Collection and storage (2018)
- Case Study: Pioneering a pan-genome reference collection (2018)