April 20, 2022
Technical overview – Iso-Seq library preparation using SMRTbell prep kit 3.0
- Tutorial
- Sequel II system, Sequel IIe system, Sequel II system and Sequel IIe system
- Library preparation, Sample preparation, Binding, Sequencing, Analysis
This technical training presentation provides an overview of Iso-Seq library sample preparation using SMRTbell prep kit 3.0 (SPK 3.0). Options for multiplexing Iso-Seq samples using barcoded cDNA primers or SMRTbell barcoded adapters are discussed. Sequencing preparation workflow details, example performance data and Iso-Seq data analysis recommendations are also presented.