Featured Documentation

Operations Guide – Sequel II and Sequel IIe Systems: The SMRT Sequencers

Description

This document describes operational procedures for Sequel II and Sequel IIe Systems. (PN: 101-774-700)

Quick Reference Card – Loading and Pre-Extension Recommendations for the Sequel II and IIe Systems

Description

This document contains loading and pre-extension recommendations for the Sequel II System.

Procedure & checklist – Amplification of bacterial full-length 16S rRNA gene with barcoded primers

Description

This document describes the PCR amplification of bacterial full-length 16S rRNA genes (V1—V9 regions) using up to 192 dual indices.

Procedure & Checklist – Preparing Multiplexed Microbial Libraries Using SMRTbell Express Template Prep Kit 2.0

Description

This streamlined procedure describes how to prepare multiplexed SMRTbell libraries of microbial genomes for sequencing on the Sequel System using the SMRTbell Express Template Preparation Kit 2.0. Up to sixteen genomes can be multiplexed on the Sequel System and up to 48 genomes can be multiplexed on the Sequel II System.

Quick Reference Card – Equipment and Materials Needed for Sequencing

Description

This Quick Reference Card lists examples of equipment and other lab materials that may be needed for sequencing on PacBio instruments.

SMRT Link user guide v11.1

Description

This document describes how to use PacBio's SMRT Link software (v11.1). SMRT Link is the web-based end-to-end workflow manager for Sequel II/IIe systems.

Guide & overview – Nanobind plant nuclei kit

Description

This document describes procedures on how to use the Nanobind Plant Nuclei Big DNA Kit for extraction of HMW (50 kb – 300+ kb) DNA from plant nuclei.

Procedure & checklist – Extracting HMW DNA from standard Dounce homogenizer tissue using Nanobind kits

Description

This procedure can be used to extract HMW DNA from animal tissues.

Procedure & checklist – Selecting fish tissue type for sequencing

Description

This procedure describes recommended methods for sequencing fish tissue.

Procedure & checklist – PacBio HiFiViral high-throughput multiplexing for full-viral genome sequencing of SARS-CoV-2 using SMRTbell prep kit 3.0

Description

This procedure can be used to capture the SARS-CoV-2 genome with tiled molecular inversion probes that create highly redundant overlapping amplicons resulting in comprehensive sequence coverage on the Sequel II and Sequel IIe Systems

Sequel SMRT Link web services API use cases (v11.0)

Description

This document describes common tasks performed using the SMRT Link (v11.0) web services API and provides "how to" recipes for accomplishing these tasks.