Documentation + protocols

This procedure can be used to construct Iso-Seq libraries from RNA for sequencing on PacBio Sequel II and IIe systems.

This procedure can be used to construct amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio Sequel II and IIe systems. Amplicons may be barcoded during PCR, during library prep with SMRTbell barcoded adapters, or by both methods to create nested barcode combinations.

This procedure can be used to prepare multiplexed AAV libraries for sequencing on the Sequel II and Sequel IIe systems. Multiplexing is achieved using SMRTbell barcoded adapters.

This procedure can be used to generate asymmetrically barcoded PCR products for library construction and SMRT sequencing by amplifying the region of interest using target-specific PCR primers tailed with M13 forward and reverse primers.

This procedure can be used to capture the SARS-CoV-2 genome with tiled molecular inversion probes that create highly redundant overlapping amplicons resulting in comprehensive sequence coverage on the Sequel II and Sequel IIe Systems

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio systems.

This procedure can be used to sequence targeted genomic regions, without amplification, using a CRISPR-Cas9 system. This protocol is applicable for the Sequel and Sequel II Systems.

This streamlined procedure describes how to prepare multiplexed SMRTbell libraries of microbial genomes for sequencing on the Sequel System using the SMRTbell Express Template Preparation Kit 2.0. Up to sixteen genomes can be multiplexed on the Sequel System and up to 48 genomes can be multiplexed on the Sequel II System.

The procedure describes a workflow for quickly constructing SMRTbell libraries from PCR products using PacBio Barcoded Overhang Adapters and SMRTbell Express Template Prep Kit 2.0. In this workflow, a barcode is introduced to each amplicon through ligation with a hairpin adapter containing a 16-bp barcode. Once barcoded, amplicons can be pooled and purified for sequencing.

This streamlined procedure can be used to prepare SMRTbell libraries using Express Template Prep Kit 2.0 and gDNA as low as 150 ng. This procedure is applicable for the Sequel and Sequel II System and enables multiplexing a maximum of 2 small genomes (up to 600 Mb/genome) on the Sequel II System.

This procedure can be used to prepare 10 kb libraries using Template Prep Kit 1.0 for generating highly accurate long reads from 1 µg sheared and concentrated DNA for metagenomic shotgun sequencing.

This procedure can be used to prepare HiFi SMRTbell libraries using ultra-low DNA input amounts.

This document describes a method for quickly constructing large insert SMRTbell libraries for generating high-accuracy long reads (HiFi reads) on the Sequel II System using the SMRTbell Express Template Prep Kit 2.0.

This document contains instructions for amplifying and constructing a SMRTbell library using Express 2.0 for sequencing full-length 16S genes on the Sequel Systems.