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Procedure & checklist – Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio systems.

Procedure & checklist – PacBio HiFiViral high-throughput multiplexing for full-viral genome sequencing of SARS-CoV-2 using SMRTbell prep kit 3.0

Description

This procedure can be used to capture the SARS-CoV-2 genome with tiled molecular inversion probes that create highly redundant overlapping amplicons resulting in comprehensive sequence coverage on the Sequel II and Sequel IIe Systems

Procedure & checklist – Preparing Iso-Seq libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct Iso-Seq libraries from RNA for sequencing on PacBio Sequel II and IIe systems.

Procedure & checklist – Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio Sequel II and IIe systems. Amplicons may be barcoded during PCR, during library prep with SMRTbell barcoded adapters, or by both methods to create nested barcode combinations.

Procedure & checklist – Preparing multiplexed AAV SMRTbell libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to prepare multiplexed AAV libraries for sequencing on the Sequel II and Sequel IIe systems. Multiplexing is achieved using SMRTbell barcoded adapters.

Procedure & Checklist – Preparing SMRTbell Libraries using PacBio Barcoded M13 Primers for Multiplex SMRT Sequencing

Description

This procedure can be used to generate asymmetrically barcoded PCR products for library construction and SMRT sequencing by amplifying the region of interest using target-specific PCR primers tailed with M13 forward and reverse primers.

Procedure & checklist – Amplification of bacterial full-length 16S rRNA gene with barcoded primers

Description

This document describes the PCR amplification of bacterial full-length 16S rRNA genes (V1—V9 regions) using up to 192 dual indices.

Procedure & Checklist – Preparing Single-Cell Iso-Seq Libraries Using SMRTbell Express Template Prep Kit 2.0

Description

This procedure can be used to construct Single-Cell Iso-Seq SMRTbell libraries using the SMRTbell Express Template Prep Kit 2.0.

Procedure & Checklist – Preparing Multiplexed Microbial Libraries Using SMRTbell Express Template Prep Kit 2.0

Description

This streamlined procedure describes how to prepare multiplexed SMRTbell libraries of microbial genomes for sequencing on the Sequel System using the SMRTbell Express Template Preparation Kit 2.0. Up to sixteen genomes can be multiplexed on the Sequel System and up to 48 genomes can be multiplexed on the Sequel II System.

Procedure & Checklist – Preparing HiFi SMRTbell Libraries from Ultra-Low DNA Input

Description

This procedure can be used to prepare HiFi SMRTbell libraries using ultra-low DNA input amounts.

Procedure & checklist – Aplysia

Description

This procedure can be used to isolate HMW DNA from the mollusk Aplysia.  

Procedure & checklist – Black mystery snail

Description

This procedure can be used to isolate HMW DNA from frozen foot tissue of black mystery snail which is a freshwater snail.

Procedure & checklist – crab muscle

Description

This procedure can be used to isolate HMW DNA from crab muscle.

Procedure & checklist – cryoPREP tissue homogenization

Description

This procedure can be used to homogenize tissue using the Covaris cryoPREP Automated Dry Pulverizer.

Procedure & checklist – Fish skeletal muscle

Description

This procedure can be used to isolate HMW DNA from fish skeletal muscle.