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Procedure checklist preparing MAS-Seq libraries using MAS-Seq for 10x 3 concatenation kit

Description

This procedure describes the workflow for constructing single-cell MAS-Seq (Multiplexed Arrays Sequencing) libraries from 10x Chromium 3’ cDNA using the MAS-Seq for 10x Single Cell 3’ kit for library prep and sequencing on PacBio systems.

Procedure & checklist – Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio Sequel II and IIe systems. Amplicons may be barcoded during PCR, during library prep with SMRTbell barcoded adapters, or by both methods to create nested barcode combinations.

Procedure & checklist – Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio systems.

Procedure & checklist – Selecting fish tissue type for sequencing

Description

This procedure describes recommended methods for sequencing fish tissue.

Procedure & checklist – Extracting HMW DNA from cultured cells using Nanobind kits

Description

This procedure can be used to extract HMW DNA from cultured cells.

Procedure & checklist – Extracting HMW DNA from fish skeletal muscle using Nanobind kits

Description

This procedure can be used to isolate HMW DNA from fish skeletal muscle.

Procedure & checklist – Extracting HMW DNA from Gram-negative bacteria using Nanobind kits

Description

This procedure can be used to extract HMW DNA from Gram-negative bacteria.

Procedure & checklist – Extracting HMW DNA from Gram-positive bacteria using Nanobind kits

Description

This procedure can be used to extract HMW DNA from Gram-positive bacteria.

Procedure & checklist – Extracting HMW DNA from mammalian whole blood using Nanobind kits

Description

This procedure can be used to extract HMW DNA from mammalian whole blood.

Procedure & checklist – Extracting HMW DNA from standard Dounce homogenizer tissue using Nanobind kits

Description

This procedure can be used to extract HMW DNA from animal tissues.

Procedure & checklist – Extracting HMW DNA from animal tissue using TissueRuptor

Description

This procedure can be used to extract HMW DNA from animal tissues using TissueRuptor.

Procedure & checklist – Extracting HMW DNA from Aplysia tissue using Nanobind kits

Description

This procedure can be used to isolate HMW DNA from the mollusk Aplysia.  

Procedure & checklist – Extracting HMW DNA from black mystery snail tissue using Nanobind kits

Description

This procedure can be used to isolate HMW DNA from frozen foot tissue of black mystery snail which is a freshwater snail.

Procedure & checklist – Extracting HMW DNA from crab muscle using Nanobind kits

Description

This procedure can be used to isolate HMW DNA from crab muscle.

Procedure & checklist – Extracting HMW DNA from human breast tissue using Nanobind kits

Description

This procedure can be used to isolate HMW DNA from fibrous human breast tissue.