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Featured Documentation

This Featured Documentation page provides the latest and most frequently used guides, protocols (procedures & checklists), and product information. See Other Documentation for product inserts, safety data sheets (SDS) and more.

March 4, 2024

Procedure & checklist — Preparing Kinnex libraries using Kinnex single-cell RNA kit

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Library preparation

Description

This procedure describes the workflow for constructing single-cell Kinnex libraries from 10x Chromium 3’ or 5’ cDNA using the Kinnex single-cell RNA kit (103-072-200) for library prep and sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.
February 23, 2024

Procedure & checklist — Extracting DNA from cultured suspension cells using Nanobind kits

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Extraction, Sample preparation

Description

This protocol describes the extraction of HMW (50–300+ kb) DNA from cultured suspension cells. It is recommended for PacBio HiFi sequencing. This protocol requires the Nanobind CBB kit (102-301-900) or the Nanobind PanDNA kit (103-260-000).
February 23, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for cultured bacteria on the Hamilton NIMBUS Presto system

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Extraction, Sample preparation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from cultured Gram-negative and Gram-positive bacteria using the Hamilton NIMBUS Presto robotic system. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
February 23, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for cultured bacteria on the KingFisher Apex system

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Extraction, Sample preparation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from cultured Gram-negative and Gram-positive bacteria using the Thermo Fisher KingFisher Apex robotic system. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
February 23, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for non human mammalian blood NHMB on the KingFisher Apex system

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Extraction, Sample preparation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 200 μL of non-human mammalian blood (NHMB) using the Thermo Fisher KingFisher Apex system. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
February 23, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for non-human mammalian blood NHMB on the Hamilton NIMBUS Presto system

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Extraction, Sample preparation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50-300 kb) DNA from 200 μL of non-human mammalian blood (NHMB) using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
February 23, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for nucleated red blood cells nRBCs on the Hamilton NIMBUS Presto system

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Extraction, Sample preparation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 5 μL of nucleated red blood cells (nRBCs) using the Hamilton NIMBUS Presto robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
February 23, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for nucleated red blood cells nRBCs on the KingFisher Apex system

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Extraction, Sample preparation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 5 μL of nucleated red blood cells (nRBCs) using the Thermo Fisher KingFisher Apex system. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
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