Featured Documentation

Procedure & checklist — Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio systems.

Procedure & checklist — Extracting HMW DNA from human whole blood using Nanobind kits

Description

This procedure can be used to extract HMW DNA from human whole blood.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Apex system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human blood using the Thermo Fisher KingFisher Apex robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Duo Prime system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Thermo Fisher KingFisher Duo Prime robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Flex system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Thermo Fisher KingFisher Flex robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the KingFisher Apex system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb DNA from 200 μL of human whole blood using the Thermo Fisher KingFisher Apex robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the KingFisher Duo Prime system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 200 μL of human whole blood using the Thermo Fisher KingFisher Duo Prime robotic instrument. This protocol requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 µL human whole blood on the KingFisher Flex system

Description

This protocol describes the extraction of HMW (50–300 kb) DNA from 200 µL of human whole blood using the Thermo Fisher KingFisher Flex. It is recommended for HiFi sequencing. This protocol requires the Nanobind HT CBB kit (102- 762-700).

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for mammalian cultured cells on KingFisher Apex system

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for mammalian cultured cells on KingFisher Duo Prime system

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for mammalian cultured cells on KingFisher Flex system

Procedure & checklist — Extracting HMW DNA from animal tissue using the Nanobind PanDNA kit

Description

This procedure can be used to extract HMW DNA from animal tissues using the Nanobind PanDNA kit

Procedure & checklist — Extracting HMW DNA from plant nuclei using Nanobind kits

Description

This procedure can be used to extract HMW DNA from plant nuclei.

Procedure & checklist — Preparing Kinnex libraries using Kinnex single-cell RNA kit

Description

This procedure describes the workflow for constructing single-cell Kinnex libraries from 10x Chromium 3’ or 5’ cDNA using the Kinnex single-cell RNA kit (103-072-200) for library prep and sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.

Procedure & checklist — Isolating nuclei from plant tissue using TissueRuptor disruption

Description

This procedure can be used to isolate nuclei from plant tissues for HMW DNA extraction using the Nanobind plant nuclei kit