Featured Documentation

Procedure & checklist — Preparing whole genome and metagenome sequencing libraries using SMRTbell prep kit 3.0

Description

This procedure can be used to construct whole-genome sequencing (WGS) libraries from genomic and metagenomic DNA using the SMRTbell prep kit 3.0 for sequencing on PacBio systems.

Procedure & checklist — Preparing Iso-Seq libraries using SMRTbell prep kit 3.0

Description

This procedure describes the workflow for constructing Iso-Seq libraries using SMRTbell prep kit 3.0 from RNA for sequencing on PacBio® Sequel® II and Revio™ systems

Procedure & checklist — Preparing Kinnex libraries from 16S rRNA amplicons

Description

This procedure provides instructions for generating Kinnex libraries from full-length 16S amplicons for sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.

Procedure & checklist — Preparing Kinnex libraries using Kinnex single-cell RNA kit

Description

This procedure describes the workflow for constructing single-cell Kinnex libraries from 10x Chromium 3’ or 5’ cDNA using the Kinnex single-cell RNA kit (103-072-200) for library prep and sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.

Procedure & checklist — Preparing Kinnex libraries using the Kinnex full-length RNA kit

Description

This procedure describes the workflow for constructing Kinnex full-length RNA libraries from total RNA samples for sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.

Procedure & checklist — Preparing multiplexed whole genome and amplicon libraries using the HiFi plex prep kit 96

Description

This procedure describes the workflow for constructing multiplexed whole genome sequencing (WGS) and amplicon libraries using the HiFi plex prep kit 96 for sequencing on PacBio systems. The HiFi plex prep kit 96 supports the multiplexing of 24 samples or more.

Procedure & checklist — Preparing whole genome libraries using the HiFi prep kit 96

Description

This procedure describes the high throughput workflow for constructing whole genome sequencing (WGS) libraries from genomic DNA using the SRE HT kit, HiFi prep kit 96 and Revio polymerase kit 96.

Procedure & checklist — Extracting HMW DNA from saliva collected in DNA Genotek Oragene devices using Nanobind kits

Description

This protocol describes the extraction of HMW DNA (50–300+ kb) from saliva collected with a DNA Genotek Oragene•DNA saliva collection device using Nanobind kits.

Procedure & checklist — Extracting HMW DNA from human whole blood using Nanobind kits

Description

This procedure can be used to extract HMW DNA from human whole blood.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Apex system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human blood using the Thermo Fisher KingFisher Apex robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Duo Prime system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Thermo Fisher KingFisher Duo Prime robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Flex system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Thermo Fisher KingFisher Flex robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the KingFisher Apex system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb DNA from 200 μL of human whole blood using the Thermo Fisher KingFisher Apex robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the KingFisher Duo Prime system

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 200 μL of human whole blood using the Thermo Fisher KingFisher Duo Prime robotic instrument. This protocol requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 µL human whole blood on the KingFisher Flex system

Description

This protocol describes the extraction of HMW (50–300 kb) DNA from 200 µL of human whole blood using the Thermo Fisher KingFisher Flex. It is recommended for HiFi sequencing. This protocol requires the Nanobind HT CBB kit (102- 762-700).