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Featured Documentation

This Featured Documentation page provides the latest and most frequently used guides, protocols (procedures & checklists), and product information. For region-specific safety data sheets (SDS), search the SDS library. For product inserts and more see Other Documentation.

December 12, 2024

Procedure & checklist — Preparing Kinnex libraries from 16S rRNA amplicons

  • Procedure & checklist
  • Revio system, Sequel IIe system, Sequel II system
  • Library preparation, Barcoding, Sequencing

Description

This procedure provides instructions for generating Kinnex libraries from full-length 16S amplicons for sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.
December 12, 2024

Procedure & checklist — Preparing Kinnex libraries using Kinnex single-cell RNA kit

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Library preparation

Description

This procedure describes the workflow for constructing single-cell Kinnex libraries from 10x Chromium 3’ or 5’ cDNA using the Kinnex single-cell RNA kit (103-072-200) for library prep and sequencing on PacBio Sequel II, Sequel IIe, and Revio systems.
December 12, 2024

Procedure & checklist — Preparing multiplexed whole genome and amplicon libraries using the HiFi plex prep kit 96

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Library preparation

Description

This procedure describes the workflow for constructing multiplexed whole genome sequencing (WGS) and amplicon libraries using the HiFi plex prep kit 96 for sequencing on PacBio systems. The HiFi plex prep kit 96 supports the multiplexing of 24 samples or more.
September 12, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Apex system

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human blood using the Thermo Fisher KingFisher Apex robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.
September 12, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Duo Prime system

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Thermo Fisher KingFisher Duo Prime robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.
September 12, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT 1 mL blood kit for human whole blood on the KingFisher Flex system

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 1 mL of human whole blood using the Thermo Fisher KingFisher Flex robotic instrument. This procedure requires the Nanobind HT 1 mL blood kit (102-762-800) and is recommended for HiFi sequencing.
September 12, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the KingFisher Apex system

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb DNA from 200 μL of human whole blood using the Thermo Fisher KingFisher Apex robotic instrument. This procedure requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
September 12, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 μL human whole blood on the KingFisher Duo Prime system

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Extraction, Automation

Description

This procedure describes the workflow for high-throughput automated extraction of HMW (50–300 kb) DNA from 200 μL of human whole blood using the Thermo Fisher KingFisher Duo Prime robotic instrument. This protocol requires the Nanobind HT CBB kit (102-762-700) and is recommended for HiFi sequencing.
September 12, 2024

Procedure & checklist — Extracting HMW DNA using the Nanobind HT CBB kit for 200 µL human whole blood on the KingFisher Flex system

  • Procedure & checklist
  • Revio system, Sequel II system and Sequel IIe system
  • Extraction, Automation

Description

This protocol describes the extraction of HMW (50–300 kb) DNA from 200 µL of human whole blood using the Thermo Fisher KingFisher Flex. It is recommended for HiFi sequencing. This protocol requires the Nanobind HT CBB kit (102- 762-700).
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