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Featured Documentation

This Featured Documentation page provides the latest and most frequently used guides, protocols, and product information. See Other Documentation for more specialized and older documentation.

October 8, 2022

Procedure & checklist – Preparing multiplexed amplicon libraries using SMRTbell prep kit 3.0

  • Procedure & Checklist
  • Sequel II System, Sequel IIe System
  • Library Preparation

Description

This procedure can be used to construct amplicon libraries using the SMRTbell prep kit 3.0 for sequencing on PacBio Sequel II and IIe systems. Amplicons may be barcoded during PCR, during library prep with SMRTbell barcoded adapters, or by both methods to create nested barcode combinations.
November 15, 2021

Procedure & Checklist – Preparing Multiplexed Microbial Libraries Using SMRTbell Express Template Prep Kit 2.0

  • Procedure & Checklist
  • Sequel IIe System, Sequel II System, Sequel System
  • Library Preparation

Description

This streamlined procedure describes how to prepare multiplexed SMRTbell libraries of microbial genomes for sequencing on the Sequel System using the SMRTbell Express Template Preparation Kit 2.0. Up to sixteen genomes can be multiplexed on the Sequel System and up to 48 genomes can be multiplexed on the Sequel II System.
July 27, 2022

Procedure & checklist – Fish tissue

  • Procedure & Checklist
  • Sequel II System:Sequel IIe System
  • Sample Preparation

Description

This procedure describes recommended methods for sequencing fish tissue.
July 27, 2022

Procedure & checklist – Aplysia

  • Procedure & Checklist
  • Sequel II System:Sequel IIe System
  • Sample Preparation

Description

This procedure can be used to isolate HMW DNA from the mollusk Aplysia.  
July 27, 2022

Procedure & checklist – Human breast

  • Procedure & Checklist
  • Sequel II System:Sequel IIe System
  • Sample Preparation

Description

This procedure can be used to isolate HMW DNA from fibrous human breast tissue.
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