July 7, 2019  |  

Twenty-one novel microsatellite loci for the endangered Florida salt marsh vole (Microtus pennsylvanicus dukecampbelli)

Authors: Austin, James D and Saarinen, Emily V and Arias-Pérez, Alberto and McCleery, Robert A and Lyons, Robert H

We present 21 microsatellite loci developed for Florida salt marsh voles (Microtus pennsylvanicus dukecampbelli). Microsatellites were identified from single molecule real time sequencing (Pacific Biosciences). We screened 30 loci and identified 21 loci as suitable for genotyping. We screened 17 individuals from Long Cabbage Key, and 3 individuals from an unnamed island. There was no significant departure from Hardy–Weinberg equilibrium or linkage equilibrium. Fifteen of the 21 loci were variable, with overall observed heterozygosity averaging 0.39, and a mean number of alleles of 3.14. Linkage disequilibrium estimate of Ne was 10.7 (95 % CI 6.1–20.1). These markers will be useful for conservation genetics studies of this endangered species.

Journal: Conservation genetics resources
DOI: 10.1007/s12686-014-0159-y
Year: 2014

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