July 7, 2019  |  

Third-generation sequencing and the future of genomics

Authors: Lee, Hayan and Gurtowski, James and Yoo, Shinjae and Nattestad, Maria and Marcus, Shoshana and Goodwin, Sara and McCombie, W. Richard and Schatz, Michael

Third-generation long-range DNA sequencing and mapping technologies are creating a renaissance in high-quality genome sequencing. Unlike second-generation sequencing, which produces short reads a few hundred base-pairs long, third-generation single-molecule technologies generate over 10,000 bp reads or map over 100,000 bp molecules. We analyze how increased read lengths can be used to address long-standing problems in de novo genome assembly, structural variation analysis and haplotype phasing.

Journal: BioRxiv
DOI: 10.1101/048603
Year: 2016

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