September 22, 2019  |  

Targeted sequencing of ampliconic gene transcripts from total human male testis RNA

Authors: Tomaszkiewicz, Marta and Makova, Kateryna

The protocol summarizes all the steps towards the construction of PacBio and Illumina sequencing libraries of transcripts from nine Y chromosome ampliconic gene families starting from total human male testis RNA from two human males. This method is based on PacBio’s Isoform Sequencing method (Iso-Seq) using the Clontech SMARTer PCR cDNA Synthesis Kit and No Size Selection with some modifications.

Journal: Protocol exchange
DOI: 10.1038/protex.2018.109
Year: 2018

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