July 7, 2019  |  

Rapid and affordable size-selected PacBio single-molecule real-time sequencing template library construction using the bead-beating DNA extraction method

Authors: Kato, Kengo and Hashino, Masanori and Ito, Tamaki and Matsui, Mari and Suzuki, Satowa and Kai, Kumiko and Kitazume, Miwako and Sekizuka, Tsuyoshi and Kuroda, Makoto

This study demonstrated that bead-beating method facilitates a simple and rapid protocol for genomic DNA isolation for Pacific BioSciences (PacBio) sequencing with library construction of sufficient length. The protocol may also be beneficial for inactivating pathogens by simultaneous and instant DNA fragmentation, with no special equipment required to obtain large DNA fragments. This protocol was comparable in terms of quality to the standard protocol suggested by PacBioand represents an alternative, rapid shortcut for performing accurate PacBio sequencing.

Journal: Journal of biological methods
DOI: 10.14440/jbm.2017.169
Year: 2017

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