April 21, 2020  |  

Plasmid-encoded tet(X) genes that confer high-level tigecycline resistance in Escherichia coli.

Authors: Sun, Jian and Chen, Chong and Cui, Chao-Yue and Zhang, Yan and Liu, Xiao and Cui, Ze-Hua and Ma, Xiao-Yu and Feng, Youjun and Fang, Liang-Xing and Lian, Xin-Lei and Zhang, Rong-Min and Tang, You-Zhi and Zhang, Kou-Xing and Liu, Han-Mian and Zhuang, Zhi-Hui and Zhou, Shi-Dan and Lv, Jing-Nan and Du, Hong and Huang, Bin and Yu, Fang-You and Mathema, Barun and Kreiswirth, Barry N and Liao, Xiao-Ping and Chen, Liang and Liu, Ya-Hong

Tigecycline is one of the last-resort antibiotics to treat complicated infections caused by both multidrug-resistant Gram-negative and Gram-positive bacteria1. Tigecycline resistance has sporadically occurred in recent years, primarily due to chromosome-encoding mechanisms, such as overexpression of efflux pumps and ribosome protection2,3. Here, we report the emergence of the plasmid-mediated mobile tigecycline resistance mechanism Tet(X4) in Escherichia coli isolates from China, which is capable of degrading all tetracyclines, including tigecycline and the US FDA newly approved eravacycline. The tet(X4)-harbouring IncQ1 plasmid is highly transferable, and can be successfully mobilized and stabilized in recipient clinical and laboratory strains of Enterobacteriaceae bacteria. It is noteworthy that tet(X4)-positive E.?coli strains, including isolates co-harbouring mcr-1, have been widely detected in pigs, chickens, soil and dust samples in China. In vivo murine models demonstrated that the presence of Tet(X4) led to tigecycline treatment failure. Consequently, the emergence of plasmid-mediated Tet(X4) challenges the clinical efficacy of the entire family of tetracycline antibiotics. Importantly, our study raises concern that the plasmid-mediated tigecycline resistance may further spread into various ecological niches and into clinical high-risk pathogens. Collective efforts are in urgent need to preserve the potency of these essential antibiotics.

Journal: Nature microbiology
DOI: 10.1038/s41564-019-0496-4
Year: 2019

Read Publication

Talk with an expert

If you have a question, need to check the status of an order, or are interested in purchasing an instrument, we're here to help.