July 7, 2019  |  

Multi-omics approach to study global changes in a triclosan-resistant mutant strain of Acinetobacter baumannii ATCC 17978.

Authors: Fernando, Dinesh M and Chong, Patrick and Singh, Manu and Spicer, Victor and Unger, Mark and Loewen, Peter C and Westmacott, Garrett and Kumar, Ayush

Acinetobacter baumannii AB042, a triclosan-resistant mutant strain, was examined for modulated gene expression using whole-genome sequencing, transcriptomics and proteomics in order to understand the mechanism of triclosan resistance as well as its impact on A. baumannii. Data revealed modulated expression of the fatty acid metabolism pathway, co-factors known to play a role in the synthesis of fatty acids, as well as several transcriptional regulators. The membrane composition of the mutant revealed a decrease in C18 with a corresponding increase in C16 fatty acids compared with the parent strain A. baumannii ATCC 17978. These data indicate that A. baumannii responds to triclosan by altering the expression of genes involved in fatty acid metabolism, antibiotic resistance and amino acid metabolism. Copyright © 2016 Elsevier B.V. and International Society of Chemotherapy. All rights reserved.

Journal: International journal of antimicrobial agents
DOI: 10.1016/j.ijantimicag.2016.10.014
Year: 2017

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