September 22, 2019  |  

Improved performance of the PacBio SMRT technology for 16S rDNA sequencing.

Authors: Mosher, Jennifer J and Bowman, Brett and Bernberg, Erin L and Shevchenko, Olga and Kan, Jinjun and Korlach, Jonas and Kaplan, Louis A

Improved sequencing accuracy was obtained with 16S amplicons from environmental samples and a known pure culture when upgraded Pacific Biosciences (PacBio) hardware and enzymes were used for the single molecule, real-time (SMRT) sequencing platform. The new PacBio RS II system with P4/C2 chemistry, when used with previously constructed libraries (Mosher et al., 2013) surpassed the accuracy of Roche/454 pyrosequencing platform. With accurate read lengths of >1400 base pairs, the PacBio system opens up the possibility of identifying microorganisms to the species level in environmental samples. Copyright © 2014 Elsevier B.V. All rights reserved.

Journal: Journal of microbiological methods
DOI: 10.1016/j.mimet.2014.06.012
Year: 2014

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