July 19, 2019  |  

High-Throughput Single-Cell Sequencing of both TCR-ß Alleles.

Authors: Hosoya, Tomonori and Li, Hongyang and Ku, Chia-Jui and Wu, Qingqing and Guan, Yuanfang and Engel, James Douglas

Allelic exclusion is a vital mechanism for the generation of monospecificity to foreign Ags in B and T lymphocytes. In this study, we developed a high-throughput barcoded method to simultaneously analyze the VDJ recombination status of both mouse TCR-ß alleles in hundreds of single cells using next-generation sequencing. Copyright © 2018 by The American Association of Immunologists, Inc.

Journal: Journal of immunology
DOI: 10.4049/jimmunol.1800774
Year: 2018

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